ELISA Kit for Osteoglycin (OGN)

OIF; SLRR3A; Mimecan Proteoglycan; Osteoinductive Factor

Specificity

This assay has high sensitivity and excellent specificity for detection of Osteoglycin (OGN).
No significant cross-reactivity or interference between Osteoglycin (OGN) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Osteoglycin (OGN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Osteoglycin (OGN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
Bone. Association of osteoglycin and FAM5C with bone turnover markers, bone mineral density, and vertebral fractures in postmenopausal women with type 2 diabetes mellitus. pubmed:27836731
Tohoku journal of experimental medicine Higher Serum Levels of Osteoglycin Are Associated with All-Cause Mortality and Cardiovascular and Cerebrovascular Events in Patients with Advanced Chronic Kidney Disease. pubmed:28824047
Molecular Metabolism Osteoglycin, a novel coordinator of bone and glucose homeostasis Pubmed:29799418
Journal of Experimental & Clinical Cancer Research Osteoglycin (OGN) reverses epithelial to mesenchymal transition and invasiveness in colorectal cancer via EGFR/Akt pathway Pubmed:29499765
Journal of Human Hypertension Association of SLRPs with carotid artery atherosclerosis in essential hypertensive patients Pubmed:29867136
Cell Reports Transforming Growth Factor-β3 Regulates Adipocyte Number in Subcutaneous White Adipose Tissue Pubmed: 30332637
J Clin Endocrinol Metab Osteoglycin across the adult lifespan 34850904
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPC688Mu02 Recombinant Osteoglycin (OGN) Positive Control; Immunogen; SDS-PAGE; WB.
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PAC688Mu01 Polyclonal Antibody to Osteoglycin (OGN) WB; IHC; ICC; IP.
PAC688Mu02 Polyclonal Antibody to Osteoglycin (OGN) WB; IHC; ICC; IP.
LAC688Mu71 Biotin-Linked Polyclonal Antibody to Osteoglycin (OGN) WB; IHC; ICC.
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LMC688Mu Multiplex Assay Kit for Osteoglycin (OGN) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.