ELISA Kit for Stanniocalcin 2 (STC2)

STCRP; Stanniocalcin-related protein

Specificity

This assay has high sensitivity and excellent specificity for detection of Stanniocalcin 2 (STC2).
No significant cross-reactivity or interference between Stanniocalcin 2 (STC2) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Stanniocalcin 2 (STC2) and the recovery rates were calculated by comparing the measured value to the expected amount of Stanniocalcin 2 (STC2) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 95-104 99
EDTA plasma(n=5) 86-94 90
heparin plasma(n=5) 78-90 82

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Stanniocalcin 2 (STC2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Stanniocalcin 2 (STC2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Stanniocalcin 2 (STC2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 96-103% 79-101% 87-101% 78-89%
EDTA plasma(n=5) 89-97% 81-99% 90-98% 89-99%
heparin plasma(n=5) 99-105% 96-103% 94-103% 97-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
International Journal of Cancer A preclinical algorithm of soluble surrogate biomarkers that correlate with therapeutic inhibition of the MET oncogene in gastric tumors Wiley: source
Canadian Journal of Physiology and Pharmacology Stanniocalcin 2 induces oxaliplatin resistance in colorectal cancer cells by upreCavia (Guinea pig )lating P-glycoprotein Pubmed:27245421
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPF913Hu01 Recombinant Stanniocalcin 2 (STC2) Positive Control; Immunogen; SDS-PAGE; WB.
PAF913Hu01 Polyclonal Antibody to Stanniocalcin 2 (STC2) WB; IHC; ICC; IP.
MAF913Hu22 Monoclonal Antibody to Stanniocalcin 2 (STC2) WB; IHC; ICC; IP.
MAF913Hu27 Monoclonal Antibody to Stanniocalcin 2 (STC2) WB; IHC; ICC; IP.
MAF913Hu29 Monoclonal Antibody to Stanniocalcin 2 (STC2) WB; IHC; ICC; IP.
MAF913Hu21 Monoclonal Antibody to Stanniocalcin 2 (STC2) WB; IHC; ICC; IP.
MAF913Hu23 Monoclonal Antibody to Stanniocalcin 2 (STC2) WB; IHC; ICC; IP.
MAF913Hu25 Monoclonal Antibody to Stanniocalcin 2 (STC2) WB; IHC; ICC; IP.
MAF913Hu24 Monoclonal Antibody to Stanniocalcin 2 (STC2) WB; IHC; ICC; IP.
SEF913Hu ELISA Kit for Stanniocalcin 2 (STC2) Enzyme-linked immunosorbent assay for Antigen Detection.
LMF913Hu Multiplex Assay Kit for Stanniocalcin 2 (STC2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSF913Hu01 ELISA Kit DIY Materials for Stanniocalcin 2 (STC2) Main materials for "Do It (ELISA Kit) Yourself".