Gene is the basis of molecular biology. Plasmid construction always includes primer design, template preparation, PCR amplification, fragment recovery, enzyme digestion and ligation, transformation and the final sequencing. The whole process usually takes two to three weeks to complete. However, for each specific gene fragment, many factors should be considered such as restriction enzyme sites. Non-specific bands should also be avoided including Hairpin, False priming and Cross Dimer formed by sense/antisense-primer. In addition, annealing temperature and reagent ratio system should be optimized though repeated tests. RNA extraction and reverse transcription of cDNA are needed for t......2013-07-24

Calprotectin, usually known as S100A8/A9, is a heterodimeric complex composed by two components, S100A8 and Mature collagen molecule has a structure of 3 short non-helix peptide chains at its N-terminal (N terminal) and carboxyl terminal (C terminal). It is called terminal peptide (Telopeptide). Type I collagen cross-linked N-telopeptide (NTX) and type I collagen cross-linked C-terminal peptide (CTX) are widely used as markers for collagen degradation. Both of NTX and CTX can be detected in serum and urine. Wherein the urinary NTX (Cross Linked N-Telopeptide Of Type I Collagen, cE90639Hu) is the direct product of type I collagen degradation by osteoclast. Not only could NTX be used t......2013-07-09

Recombinant protein technology is one of the most widely used biotechnology to obtain target protein. Protein purification is the most critical and difficult step in recombinant protein technology. The quality of the target proteins depends on the step of purification. Currently, the conventional method is to fuse target protein and an affinity purification label (Tag), the target protein can be purified through affinity purification of the tag. In addition, how to detect the expression of target protein is also a big problem for biologists. It is obviously complicated and expensive to use different antibodies to detect different target proteins. Sometimes it is even impossible to f......2013-07-09

Advanced Glycation End products (AGEs) is a general term for series of stable and highly active end-end products formed by non-enzymatic glycosylation reaction. The structure of AGE is highly heterogeneous. The accumulation of AGE in the body would cause various complications from diabetes. Recent studies suggest that advanced glycation end products (AGEs) would lead to defects in insulin secretion and the development of type 1 diabetes through damage to mitochondrial function. As a new clinical indicator, AGEs detection is valuable for investigating the mechanisms of derangement of glucose metabolism and estimation of treatment effect. Due to acid instability and complex structure ......2013-07-04

17-hydroxyprogesterone Detection Kit 17-hydroxyprogesterone ( 17-OHP) is an endogenous hormone secreted by the adrenal and gonadal. It is mainly converted by progesterone or 17- hydroxypregnenolone. 17-OHP plays an important role in organ development. 17 hydroxyl progesterone content in serum is the main indicator for the diagnosis of congenital adrenal hyperplasia. The current method to detect 17 hydroxyl progesterone includes: radioimmunoassay, immunofluorescence, mass spectrometry and enzyme-linked immunosorbent assay. Compared to immunofluorescence and mass spectrometry in high demands of equipment, enzyme linked immunosorbent assay (ELISA) has advantages of harmlessness and ea......2013-06-15

1. Principle Chemiluminescence Kit (CLIA Kit) is a kind of sensitive Kit which uses Streptavidin-HRP as enzyme and enhanced ECL chemiluminescent system as substrate reagent. The assay processes of CLIA Kit is listed below: at first, test samples are added into black opaque microplates which were coated with antibodies; secondly, horseradish peroxidase complex conjugates with specific antibody was added, and then, the un-reacted ingredients were washed away. Thirdly, Luminol chemiluminescent substrate will be added into the microwells, and then relative luminosity values (RLU) will be scanned by photon counter reader, the detection principle of the Kit is showed in Figure 1. Ch......2013-05-29