Recombinant protein technology is one of the most widely used biotechnology to obtain target protein. Protein purification is the most critical and difficult step in recombinant protein technology. The quality of the target proteins depends on the step of purification. Currently, the conventional method is to fuse target protein and an affinity purification label (Tag), the target protein can be purified through affinity purification of the tag. In addition, how to detect the expression of target protein is also a big problem for biologists. It is obviously complicated and expensive to use different antibodies to detect different target proteins. Sometimes it is even impossible to f......2013-07-09

Advanced Glycation End products (AGEs) is a general term for series of stable and highly active end-end products formed by non-enzymatic glycosylation reaction. The structure of AGE is highly heterogeneous. The accumulation of AGE in the body would cause various complications from diabetes. Recent studies suggest that advanced glycation end products (AGEs) would lead to defects in insulin secretion and the development of type 1 diabetes through damage to mitochondrial function. As a new clinical indicator, AGEs detection is valuable for investigating the mechanisms of derangement of glucose metabolism and estimation of treatment effect. Due to acid instability and complex structure ......2013-07-04

17-hydroxyprogesterone Detection Kit 17-hydroxyprogesterone ( 17-OHP) is an endogenous hormone secreted by the adrenal and gonadal. It is mainly converted by progesterone or 17- hydroxypregnenolone. 17-OHP plays an important role in organ development. 17 hydroxyl progesterone content in serum is the main indicator for the diagnosis of congenital adrenal hyperplasia. The current method to detect 17 hydroxyl progesterone includes: radioimmunoassay, immunofluorescence, mass spectrometry and enzyme-linked immunosorbent assay. Compared to immunofluorescence and mass spectrometry in high demands of equipment, enzyme linked immunosorbent assay (ELISA) has advantages of harmlessness and ea......2013-06-15

1. Principle Chemiluminescence Kit (CLIA Kit) is a kind of sensitive Kit which uses Streptavidin-HRP as enzyme and enhanced ECL chemiluminescent system as substrate reagent. The assay processes of CLIA Kit is listed below: at first, test samples are added into black opaque microplates which were coated with antibodies; secondly, horseradish peroxidase complex conjugates with specific antibody was added, and then, the un-reacted ingredients were washed away. Thirdly, Luminol chemiluminescent substrate will be added into the microwells, and then relative luminosity values (RLU) will be scanned by photon counter reader, the detection principle of the Kit is showed in Figure 1. Ch......2013-05-29

Why we need the High Sensitive ELISA Kits ? In the processes of biological products preparation, it is inevitable to use BSA, OVA or ALB as protection protein or carrier protein, hence, the residue detection is critical for the quality of biological products; besides, residue detection were also broadly utilized to determine the metabolism and effect of the medicine in Medical and Physiology research, for instance, salmon calcitonin detection, etc. Generally, the concentration of residue is very low, some of them are just at the level of pg/ml, so, it is quite difficult for detection. Based on this situation, higher sensitive detection Kits are needed. Cloud-Clone Corp. have develo......2013-05-20

Lipopolysaccharide (Lipopolysaccharides ,LPS) is one of the main components of Gram-negative bacteria, which is also known as endotoxin in traditional idea. LPS can cause fever, shock, and even lead to death. With growing popularity of genetic engineering techniques in biological products and reagents, LPS becomes an important indicator to monitor residual bacteria as well as pyrogenicity in bio-products. Cloud-Clone Corp. utilized special methods that can greatly enhance LPS immunogenicity, and monoclonal antibodies(MAB526Ge22) with high specificity against LPS was also obtained by hybridoma technique. The LPS detection Kit (CEB526Ge) was developed on this basis. 88 samples run a......2013-05-07