Packages (Simulation)
Image (II)
-
Figure. SDS-PAGE
Quality Guarantee
Instruction Manual
Certificate
Active Heat Shock Protein 70 (HSP70)
DnaK; 70 Kilodalton Heat Shock Proteins
- Product No.APA873Hu61
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 90%
- Isoelectric Point5.3
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$
US$
US$
US$
US$
For more details, please contact local distributors!
ACTIVITY TEST
Heat Shock Protein 70 (HSP70) is a vital and highly conserved protein. It exists in organisms from bacteria to humans. Under normal conditions, it aids in protein folding and maintenance of cellular proteostasis. When cells encounter stress like heat or toxins, HSP70 expression surges. It then prevents protein aggregation, assists in refolding damaged proteins, and plays a key role in cell survival and stress response, safeguarding cellular function and integrity.Besides,The activation of HSP70 protein depends on the hydrolysis of ATP, which can produce inorganic Pi phosphate. Thus,the activity of recombinant human HSP70 was measured by its ability to hydrolyze the substrate ATP to phosphate which was detected by the malachite green phosphate detection Kit (Beyotime # S0196M). The reaction was performed in the assay buffer 20 mM HEPES, 13 mM NaCl and 1% glycerol, pH 8.5, initiated by addition 27 μL of various concentrations of HSP70 (diluted by assay buffer) to 3 µL of 100 mM ATP and 100 mM MgCl2 mixture. The final well serves as a negative control with no HSP70, replaced with 27 μL assay buffer. Incubated at 37℃ for 5min, then stop the reaction by quickly freezing samples in the dry ice and ethanol. 4 ul samples was added to 196 ul assay buffer after thawing at room temperature followed by adding 70 ul phosphate detection agent and incubated at room temperature for 30min, then read at a wavelength of 630 nm. The specific activity of recombinant human HSP70 is >12000 pmol/min/µg.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
| Magazine | Citations |
| Journal of Molecular Medicine | Proteomic analysis reveals significant elevation of heat shock protein 70 in patients with chronic heart failure due to arrhythmogenic right ventricular cardiomyopathy SpringerLink: b8738j3571287425 |
| Journal of Neuroinflammation | Neuroinflammation in autism spectrum disorders PubMed: PMC3549857 |
| Eur Arch Psychiatry Clin Neurosci. | Luteal serum BDNF and HSP70 levels in women with premenstrual dysphoric disorder Pubmed: 23455589 |
| Arch Bronconeumol | Concentraciones de proteínas de estrés térmico, L-arginina y dimetilarginina asimétrica en pacientes con síndrome de apnea obstructiva del sue PubMed: 26055465 |
| Arch Bronconeumol | Heat Shock Proteins, PubMed: 26055465 |
| Animal Science Journal | The effect of heat stress on gene expression and synthesis of heat‐shock and milk proteins in bovine mammary epithelial cells PubMed: 26467738 |
| Transplant Proc | L-Alanyl-Glutamine Attenuates Oxidative Stress in Liver Transplantation Patients PubMed: 26518955 |
| Plos One | Long-Term Effects of Induced Hypothermia on Local and Systemic Inflammation-Results from a Porcine Long-Term Trauma Model Pubmed:27144532 |
| J Pak Med Assoc. | Elevated interictal serum HSP-70 levels as an indicator of neurodegeneration for chronic migraine mnstemps:33-1429777310 |
| Egyptian Poultry Science Journal | STUDY THE RELATIONSHIP BETWEEN SELENIUM AND HEAT SHOCK PROTEINS UNDER HEAT STRESS FOR LOCAL SINAI CHICKENS STRAIN abstract:3d115259031 |
| Physiological consequences for rainbow trout (Oncorhynchus mykiss) of short-term exposure to increased suspended solid load S0144860916301935 | |
| Animal Science Journal | Prokaryotic expression of chicken interferon-γ fusion protein and its effect on expression ofpoultry heat shock protein 70 under heat stress. pubmed:27753175 |
| Pharmacol Rep | High-dose testosterone enanthate supplementation boosts oxidative stress, but exerts little effect on the antioxidant barrier in sedentary adolescent male rat liver. pubmed:28531762 |
| Biomed Pharmacother. | SIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from death. pubmed:28122311 |
| Immunobiology | Concentrations of SP-A and HSP70 are associated with polarization of macrophages in pleural effusions of non-small cell lung cancer pubmed:29111316 |
| Egyptian Poultry Science Journal | EFFECT OF DIETARY SOURCE OF SELENIUM ON PRODUCTIVE AND REPRODUCTIVE PERFORMANCE OF SINAI LAYING HENS UNDER HEAT STRESS CONDITIONS. 125788660 |
| Biomedicine & Pharmacotherapy | SIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from death DOI:10.1016/j.biopha.2017.01.087 |
| Egyptian Poultry Science Journal | IMPACT OF CHROMIUM PICOLINATE SUPPLEMENTATION ON PRODUCTIVE PERFORMANCE, IMMUNE RESPONSE AND HEAT SHOCK PROTEINS OF BROILER CHICKENS UNDER HEAT-STRESS CONDITION. ISSN: 1110-5623 |
| Aquaculture | Physiological consequences of chronic exposure of rainbow trout (Oncorhynchus mykiss) to suspended solid load in recirculating aquaculture systems 10.1016/j.aquaculture.2017.11.030 |
| Journal of dairy research | Detection of 70 kDa heat shock protein in the saliva of dairy cows. pubmed:28625184 |
| Journal of animal science | Reducing rumen starch fermentation of wheat with three percent sodium hydroxide has the potential to ameliorate the effect of heat stress in grain-fed wethers pubmed:29293755 |
| Biochemistry and Cell Biology | Ectopic expression of Miro 1 ameliorates seizures and inhibits hippocampal neurodegeneration in a mouse model of pilocarpine epilepsy Pubmed:29365285 |
| Egyptian Poultry Science Journal | THE EFFECT OF CHROMIUM PICOLINATE SUPPLEMENTATION ON IMMUNE RESPONSE AND HEAT SHOCK PROTEINS OF LAYING HENS UNDER HEAT … article_5570_5c06907ac09a7724a58d2b796fe91aba.pdf |
| Egyptian Poultry Science Journal | EFFECT OF MITIGATION OF HEAT STRESS BY EARLY HEATE ACCLIMATION AND GLUTAMINE INJECTION ON SOME PHYSIOLOGICAL MEASUREMENTS … : |
| FASEB Journal | Signature-oriented investigation of the efficacy of multicomponent drugs against heart failure Pubmed: 30230922 |
| Journal of Biological Sciences | Maged Mohamed Abd-Elhakim Barakat, Noha Ahmed El-Boghdady, Ebtehal Kamal Eldin Farrag, Ataa Abdelhalim Said and Sylvia Edward Shaker |
| Biological Procedures Online | Effective Visualization and Easy Tracking of Extracellular Vesicles in Glioma Cells |
| Journal of Biological Sciences | Protective and Curative Effects of Bombax ceiba Flower and Ziziphus spina christi Fruit Extracts on Gastric Ulcer |
| 博士论文 | PHYSIOLOGICAL ADAPTATIONS TO HEAT ACCLIMATION; REPERCUSSIONS ON CYCLING PERFORMANCE |
| environmental science and pollution research | Possible prophylactic effect of omega-3 fatty acids on cadmium-induced neurotoxicity in rats' brains Pubmed: 31468353 |
| ACTA TROPICA | Oxidative stress mediated apoptotic potential of mefloquine on experimental trichinellosis Pubmed: 33221280 |
| BioMed Research International | Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress Pubmed: 32685488 |
| Slovak Journal of Animal Science | EFFECTS OF HEAT STRESS ON PHYSIOLOGICAL PARAMETERS AND SERUM CONCENTRATION OF HSP70 IN INDIGENOUS BREEDS OF SHEEP IN NIGERIA |
| 博士论文 | Effect of organic acid blend and prebiotic on growth performance gut histomorphometry, tibia bone characteristics, reactive oxygen species, microbial count and … |
| Translational Medicine Communications | Concurrent high-intensity aerobic and resistance exercise modulates systemic release of alarmins (HMGB1, S100A8/A9, HSP70) and inflammatory … |
| Physiological impacts of suspended solids in recirculating aquaculture | |
| Clin Oral Investig | Evaluation of heat shock protein 70 and toll-like receptor 4 expression in gingival crevicular fluid in response to orthodontic forces 34091792 |
| Journal of Thermal Biology | Effects of L-proline on cellular responses of hen erythrocytes subjected to thermal stress 33627283 |
| J Korean Neurosurg Soc | The Restorative Effect of Gallic Acid on the Experimental Sciatic Nerve Damage Model 34376039 |
| PLoS One | Neuroprotective effect of sodium alginate against chromium-induced brain damage in rats Pubmed:35421180 |
| Med Science | Identification of marker proteins in preterm and term birth: Relationship of rankl and HSP70 in the cord blood |