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Active Spike Protein (SP)
S Protein
- Product No.APX273Ge01
- Organism SpeciesPan-species (General) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder
- Purity> 90%
- Isoelectric Point8.7
- ApplicationsCell culture; Activity Assays.
- Downloadn/a
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
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For more details, please contact local distributors!
ACTIVITY TEST
The coronavirus spike (S) glycoprotein is a class I viral fusion protein on the outer envelope of the virion that plays a critical role in viral infection by recognizing host cell receptors and mediating fusion of the viral and cellular membranes. The S proteins consist of two subunits, S1 and S2.Be-
-sides,S1 binding to ACE2 and CLEC4M/DC-SIGNR receptors and internalizationof thevirus into t-
-he endosomes of the host cell induces conformational changes in the S glycoprotein. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human ACE2 and recombinant Spike glycoprotein(S1). Briefly, S1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to ACE2-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-S1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of recombinant Spike glycoprotein(S1) and recombinant human ACE2 was shown in Figure 1, the EC50 for this effect is 0.034µg/mL.
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Catalog No. | Related products for research use of Pan-species (General) Organism species | Applications (RESEARCH USE ONLY!) |
| APX273Ge02 | Active Spike Protein (SP) | Cell culture; Activity Assays. |
| APX273Ge01 | Active Spike Protein (SP) | Cell culture; Activity Assays. |
| RPX273Ge01 | Recombinant Spike Protein (SP) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPX273Ge02 | Recombinant Spike Protein (SP) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPX273Ge03 | Recombinant Spike Protein (SP) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAX273Ge01 | Polyclonal Antibody to Spike Protein (SP) | IHC |
| RAX273Ge21 | Recombinant Antibody to Spike Protein (SP) | WB; IF; ICC; IHC; IP; FCM. |