ELISA Kit for Androstenedione (ASD)
- Product No.CEA456Ge
- Organism SpeciesPan-species (General) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range123.5-10,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 44.2pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! 796 US$ 796
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For more details, please contact local distributors! US$ 55692
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This assay has high sensitivity and excellent specificity for detection of Androstenedione (ASD).
No significant cross-reactivity or interference between Androstenedione (ASD) and analogues was observed.
Matrices listed below were spiked with certain level of Androstenedione (ASD) and the recovery rates were calculated by comparing the measured value to the expected amount of Androstenedione (ASD) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Androstenedione (ASD) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Androstenedione (ASD) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Androstenedione (ASD) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|Reagent Diluent||1×300µL||Stop Solution||1×6mL|
|TMB Substrate||1×9mL||Instruction manual||1|
|Wash Buffer (30 × concentrate)||1×20mL|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|Plos One||Association between Polycystic Ovary Syndrome and Cavia (Guinea pig )t Microbiota Pubmed:27093642|
|Scientific Reports||Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus pubmed:27461373|
|33||Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome. pubmed:28336389|
|Journal of Endocrinology||Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome Pubmed: 29535146|
|International Journal of Biological Sciences||Melatonin Stimulates STAR Expression and Progesterone Production via Activation of the PI3K/AKT Pathway in Bovine Theca Cells|
|Molecular reproduction and development||Evidence that downregulation of Wilms' tumor 1 (WT1) is involved in cortical stromal cell differentiation into theca cells in adult bovine ovaries Pubmed: 31490589|
|DOMESTIC ANIMAL ENDOCRINOLOGY||Wilms' tumor (WT1)(+/-KTS) variants decreases the progesterone secretion of bovine ovarian theca cells Pubmed: 32739762|
|ANIMAL REPRODUCTION SCIENCE||A study on steroidogenic elaborations of stroma and their regulation in response to ovarian hormones in goats 33845412|
|Reprod Domest Anim||Extracellular Vesicles of Bovine Small Follicular Fluid Promote Ovarian Cortical Stromal Cell Proliferation and Steroidogenesis 34402549|
|Curr Oncol||Plasma Aromatase Activity Index, Gonadotropins and Estrone Are Associated with Frailty Syndrome in Post-Menopausal Women with Breast Cancer Pubmed:35323344|
|Catalog No.||Related products for research use of Pan-species (General) Organism species||Applications (RESEARCH USE ONLY!)|
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|KSA456Ge11||ELISA Kit DIY Materials for Androstenedione (ASD)||Main materials for "Do It (ELISA Kit) Yourself".|