ELISA Kit DIY Materials for Androstenedione (ASD)

4-Androstenedione; 4-Androstene-3,17-Dione


The Abs in the kit have high sensitivity and excellent specificity for detection of Androstenedione (ASD). No significant cross-reactivity or interference between Androstenedione (ASD) and analogues was observed.


1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.


Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support Pack



Magazine Citations
Plos One Association between Polycystic Ovary Syndrome and Cavia (Guinea pig )t Microbiota Pubmed:27093642
Scientific Reports Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus pubmed:27461373
33 Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome. pubmed:28336389
Journal of Endocrinology Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome Pubmed: 29535146
International Journal of Biological Sciences Melatonin Stimulates STAR Expression and Progesterone Production via Activation of the PI3K/AKT Pathway in Bovine Theca Cells
Molecular reproduction and development Evidence that downregulation of Wilms' tumor 1 (WT1) is involved in cortical stromal cell differentiation into theca cells in adult bovine ovaries Pubmed: 31490589
DOMESTIC ANIMAL ENDOCRINOLOGY Wilms' tumor (WT1)(+/-KTS) variants decreases the progesterone secretion of bovine ovarian theca cells Pubmed: 32739762
ANIMAL REPRODUCTION SCIENCE A study on steroidogenic elaborations of stroma and their regulation in response to ovarian hormones in goats 33845412
Reprod Domest Anim Extracellular Vesicles of Bovine Small Follicular Fluid Promote Ovarian Cortical Stromal Cell Proliferation and Steroidogenesis 34402549
Curr Oncol Plasma Aromatase Activity Index, Gonadotropins and Estrone Are Associated with Frailty Syndrome in Post-Menopausal Women with Breast Cancer Pubmed:35323344
Catalog No. Related products for research use of Pan-species (General) Organism species Applications (RESEARCH USE ONLY!)
CPA456Ge11 BSA Conjugated Androstenedione (ASD) Immunogen; SDS-PAGE; WB.
CPA456Ge21 OVA Conjugated Androstenedione (ASD) Immunogen; SDS-PAGE; WB.
PAA456Ge01 Polyclonal Antibody to Androstenedione (ASD) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
LAA456Ge71 Biotin-Linked Polyclonal Antibody to Androstenedione (ASD) WB; IHC; ICC.
CEA456Ge ELISA Kit for Androstenedione (ASD) Enzyme-linked immunosorbent assay for Antigen Detection.
IEA456Ge Instant ELISA Kit for Androstenedione (ASD) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA456Ge Magnetic Luminex Assay Kit for Androstenedione (ASD) ,etc. Magnetic Luminex Assay for Antigen Detection.
KSA456Ge11 ELISA Kit DIY Materials for Androstenedione (ASD) Main materials for "Do It (ELISA Kit) Yourself".