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Human Hela cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.2% BSA-Triton X-100,then stained with 10µg/ml mouse Anti-human MDM2 Monoclonal Antibody (Catalog FAG790Hu02, red histogram) or Isotype control antibody (Catalog IS067-Mu01, green histogram), followed by 1µg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).
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Anti-Mdm2 p53 Binding Protein Homolog (MDM2) Monoclonal Antibody
HDMX; hdm2; Mouse Double Minute 2,Human Homolog Of p53-Binding Protein; Oncoprotein Mdm2
- Product No.FAG790Hu02
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Potency (Clone Number)C12
- Ig Isotype IgG1 Kappa
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Excitation Laser
- Vol. per test
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Organism Species Moren/a
- ApplicationsFCM
- Downloadn/a
- UOM 25test50test 100test 200test 500test
- FOB
US$ 132
US$ 220
US$ 352
US$ 440
US$ 770
For more details, please contact local distributors!
USAGE
Flow cytometry: 10µg/ml;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
GIVEAWAYS
INCREMENT SERVICES
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Protein A/G Purification Column
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Isotype Control
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Real Time PCR Experimental Service
| Magazine | Citations |
| Clin Cancer Res | Inactivation of the p53–KLF4–CEBPA Axis in Acute Myeloid Leukemia PubMed: 26408402 |
| Clin Cancer Res | Inactivation of the p53–KLF6–CEBPA Axis in Acute Myeloid Leukemia PubMed: 26408402 |
| Analyst | Sensitive and simultaneous surface plasmon resonance detection of free and p53-bound MDM2 proteins from human sarcomas Pubmed:29637949 |