≡

Active Cathepsin V (CTSV)

CTS-L2; CTSL2; CATL2; CTSU; CTS-V; Cathepsin L2

ACTIVITY TEST

Cathepsin V (CTSV) is a lysosomal cysteine proteinase which belongs to peptidase C1 family. It is expressed in normal human thymus, testis and corneal epithelium. Cathepsin V plays an important role in corneal physiology and mediates degradation of invariant chain in human thymus. Besides, Retinoblastoma Protein 1 (RB1) has been identified as an interactor of CTSV, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CTSV and recombinant human RB1. Briefly, CTSV were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to RB1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CTSV pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of CTSV and RB1 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APC970Hu01 Active Cathepsin V (CTSV) Cell culture; Activity Assays.
RPC970Hu01 Recombinant Cathepsin V (CTSV) Positive Control; Immunogen; SDS-PAGE; WB.
PAC970Hu01 Polyclonal Antibody to Cathepsin V (CTSV) WB; IHC; ICC; IP.
MAC970Hu22 Monoclonal Antibody to Cathepsin V (CTSV) WB; IHC; ICC; IP.
SEC970Hu ELISA Kit for Cathepsin V (CTSV) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC970Hu Multiplex Assay Kit for Cathepsin V (CTSV) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.