High Sensitive ELISA Kit for Interleukin 35 (IL35)
- Product No.HEC008Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range1.56-100pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.54pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 770
For more details, please contact local distributors! US$ 3465
For more details, please contact local distributors! US$ 6545
For more details, please contact local distributors! US$ 53900
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of High Sensitive Interleukin 35 (IL35).
No significant cross-reactivity or interference between High Sensitive Interleukin 35 (IL35) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant High Sensitive Interleukin 35 (IL35) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Interleukin 35 (IL35) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Interleukin 35 (IL35) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Interleukin 35 (IL35) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Interleukin 35 (IL35) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Oncology Letters||Aberrant expression of Treg-associated cytokine IL-35 along with IL-10 and TGF-β in acute myeloid leukemia. PubMed: 22783403|
|Journal of Maternal-Fetal and Neonatal Medicine||Plasma IL-17, IL-35, interferon-γ, SOCS3 and TGF-β levels in pregnant women with preeclampsia, and their relation with severity of disease Pubmed: 24175856|
|Journal of Reproductive Immunology||What is the impact of Th1/Th2 ratio, SOCS3, IL17, and IL35 levels in unexplained infertility? Pubmed: 24368037|
|Human Immunology||The imbalanced profile and clinical significance of T helper associated cytokines in bone marrow microenvironment of the patients with acute myeloid leukemia ScienceDirect: S0198885913005776|
|Folia Biologica (Praha)||Gene Expression of Subunits of the IL-12 Family Cytokines in moDCs Derived In Vitro from the Cord Blood of Children of Healthy and Allergic Mothers Pubmed:24785110|
|Molecular Medicine Reports||Function of interleukin?17 and ?35 in the blood of patients with hepatitis B?related liver cirrhosis Pubmed:25323532|
|Rom J Morphol Embryol.||The regular distribution and expression pattern of immunosuppressive cytokine IL-35 in mouse uterus during early pregnancy Pubmed:25611266|
|Journal of Immunotherapy||Wogonin Inhibits Tumor-derived Regulatory Molecules by Suppressing STAT3 Signaling to Promote Tumor Immunity PubMed: 25962106|
|PLOS ONE||Interleukin 35 Synovial Fluid Levels Are Associated with Disease Activity of Rheumatoid Arthritis PubMed: 26204444|
|Rheumatology (Oxford)||Interleukin-35 is upregulated in systemic sclerosis and its serum levels are associated with early disease PubMed: 26231346|
|Cytokine||Anti-inflammatory effects of interleukin-35 in acquired aplastic anemia PubMed: 26282938|
|J Biol Chem||Interleukin-35 Inhibits Endothelial Cell Activation by Suppressing MAPK-AP-1 Pathway PubMed: 26085094|
|Br J Haematol||Interleukin 35 may contribute to the loss of immunological self‐tolerance in patients with primary immune thrombocytopenia PubMed: 25640666|
|Autoimmunity Reviews||New insights into immune mechanisms underlying response to Rituximab in patients with membranous nephropathy: A prospective study and a review of the literature Pubmed:26876383|
|Clinical research||Increased plasma concentrations of interleukin 35 in patients with coronary artery disease Journal:-19|
|Clinical Rheumatology||The clinical utility of serum IL-35 in patients with polymyositis and dermatomyositis pubmed:27502600|
|Respiratory Medicine||Enhanced LPS-induced activation of IL-27 signalling in sarcoidosis pubmed:27492538|
|International Journal of Molecular Medicine||Effects of an intravitreal injection of interleukin-35-expressing plasmid on pro-inflammatory andanti-inflammatory cytokines. pubmed:27460435|
|Reproductive Sciences||Upregulation of Interleukin 35 in Patients With Endometriosis Stimulates Cell Proliferation pubmed:28659009|
|EXPERIMENTAL AND THERAPEUTIC MEDICINE||IL‑35 may maintain homeostasis of the immune microenvironment in periodontitis issn:2542-3975|
|The Journal of Rheumatology||Synoviocytes-derived Interleukin 35 Potentiates B Cell Response in Patients with Osteoarthritis and Rheumatoid Arthritis pubmed:29247146|
|Cellular & Molecular Immunology||Human immunodeficiency virus type-1 induces a regulatory B cell-like phenotype in vitro pubmed:28713164|
|Molecular Medicine Reports||Lower level of IL‑35 and its reduced inhibition in Th17 cells in patients with bone marrow mononuclear cells Coombs test‑positive hemocytopenia. pubmed:29257310|
|Asian pacific journal of allergy and immunology||Evaluation of serum interleukin- 35 level in children with persistent asthma. pubmed:27996283|
|PLoS One||Interleukin-35 mitigates the function of murine transplanted islet cells via regulation of Treg/Th17 ratio pubmed:29236782|
|Hepatology Communications||Significant association between FOXP3 gene polymorphism and steroid-resistant acute rejection in living donor liver transplantation 10.1002/hep4.1052|
|International Journal of Clinical and Experimental Medicine||Clinical significance of IL-35 expression in the progression of clear cell renal cell carcinoma ISSN:1940-5901|
|OncoTargets and Therapy||Interleukin-35 as a predictor of prostate cancer in patients undergoing initial prostate biopsy. pubmed:28761357|
|Annals of Rheumatic Diseases||AB0734 Serum il-35 levels in systemic sclerosis and relationship with clinical features 10.1136:annrheumdis-2018-eular.2605|
|Tohoku Journal of Experimental Medicine||Interleukin-35 as a New Biomarker of Renal Involvement in Lupus Nephritis Patients Pubmed:29576585|
|Turkish Journal Of Medical Sciences||The impact of plasma SOCS3 levels and endometrial leukocytes on unexplained infertility Pubmed:29914245|
|Oncotarget||Pro-tumoral immune cell alterations in wild type and Shb-deficient mice in response to 4T1 breast carcinomas Pubmed:29721156|
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