Monoclonal Antibody to Interleukin 35 (IL35)
- Product No.MAC008Hu21
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourceMonoclonal antibody preparation
- Potency (Clone Number)B5
- Ig Isotype IgG
- PurificationProtein A + Protein G affinity chromatography
- Immunogen RPC008Hu01-Recombinant Interleukin 35 (IL35)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
- DownloadInstruction Manual
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The antibody is a mouse monoclonal antibody raised against IL35. It has been selected for its ability to recognize IL35 in immunohistochemical staining and western blotting.
Western blotting: 0.5-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-30ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml
Immunohistochemistry in paraffin section: 5-30ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
|Oncology Letters||Aberrant expression of Treg-associated cytokine IL-35 along with IL-10 and TGF-β in acute myeloid leukemia. PubMed: 22783403|
|Journal of Maternal-Fetal and Neonatal Medicine||Plasma IL-17, IL-35, interferon-γ, SOCS3 and TGF-β levels in pregnant women with preeclampsia, and their relation with severity of disease Pubmed: 24175856|
|Journal of Reproductive Immunology||What is the impact of Th1/Th2 ratio, SOCS3, IL17, and IL35 levels in unexplained infertility? Pubmed: 24368037|
|Human Immunology||The imbalanced profile and clinical significance of T helper associated cytokines in bone marrow microenvironment of the patients with acute myeloid leukemia ScienceDirect: S0198885913005776|
|Folia Biologica (Praha)||Gene Expression of Subunits of the IL-12 Family Cytokines in moDCs Derived In Vitro from the Cord Blood of Children of Healthy and Allergic Mothers Pubmed:24785110|
|Molecular Medicine Reports||Function of interleukin?17 and ?35 in the blood of patients with hepatitis B?related liver cirrhosis Pubmed:25323532|
|Rom J Morphol Embryol.||The regular distribution and expression pattern of immunosuppressive cytokine IL-35 in mouse uterus during early pregnancy Pubmed:25611266|
|Journal of Immunotherapy||Wogonin Inhibits Tumor-derived Regulatory Molecules by Suppressing STAT3 Signaling to Promote Tumor Immunity PubMed: 25962106|
|PLOS ONE||Interleukin 35 Synovial Fluid Levels Are Associated with Disease Activity of Rheumatoid Arthritis PubMed: 26204444|
|Rheumatology (Oxford)||Interleukin-35 is upregulated in systemic sclerosis and its serum levels are associated with early disease PubMed: 26231346|
|Cytokine||Anti-inflammatory effects of interleukin-35 in acquired aplastic anemia PubMed: 26282938|
|J Biol Chem||Interleukin-35 Inhibits Endothelial Cell Activation by Suppressing MAPK-AP-1 Pathway PubMed: 26085094|
|Br J Haematol||Interleukin 35 may contribute to the loss of immunological self‐tolerance in patients with primary immune thrombocytopenia PubMed: 25640666|
|Autoimmunity Reviews||New insights into immune mechanisms underlying response to Rituximab in patients with membranous nephropathy: A prospective study and a review of the literature Pubmed:26876383|
|Clinical research||Increased plasma concentrations of interleukin 35 in patients with coronary artery disease Journal:-19|
|Clinical Rheumatology||The clinical utility of serum IL-35 in patients with polymyositis and dermatomyositis pubmed:27502600|
|Respiratory Medicine||Enhanced LPS-induced activation of IL-27 signalling in sarcoidosis pubmed:27492538|
|International Journal of Molecular Medicine||Effects of an intravitreal injection of interleukin-35-expressing plasmid on pro-inflammatory andanti-inflammatory cytokines. pubmed:27460435|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|RPC008Hu01||Recombinant Interleukin 35 (IL35)||Positive Control; Immunogen; SDS-PAGE; WB.|
|APC008Hu01||Active Interleukin 35 (IL35)||Cell culture; Activity Assays.|
|PAC008Hu01||Polyclonal Antibody to Interleukin 35 (IL35)||WB; IHC; ICC; IP.|
|MAC008Hu21||Monoclonal Antibody to Interleukin 35 (IL35)||WB; IHC; ICC; IP.|
|LAC008Hu72||Biotin-Linked Monoclonal Antibody to Interleukin 35 (IL35)||WB; IHC; ICC.|
|SEC008Hu||ELISA Kit for Interleukin 35 (IL35)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|HEC008Hu||High Sensitive ELISA Kit for Interleukin 35 (IL35)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|PSC008Hu01||Antibody Pair for Interleukin 35 (IL35)||ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.|
|KSC008Hu01||ELISA Kit DIY Materials for Interleukin 35 (IL35)||Main materials for "Do It (ELISA Kit) Yourself".|