ELISA Kit DIY Materials for Interleukin 35 (IL35)
- Product No.KSC008Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range1.56-100pg/mL
- Applicable Sensitivity
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- DownloadInstruction Manual
96T*20 96T*50 96T*100
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For negotiated price and more details, please contact local distributors! US$ 12600
For negotiated price and more details, please contact local distributors!
The Abs in the kit have high sensitivity and excellent specificity for detection of Interleukin 35 (IL35). No significant cross-reactivity or interference between Interleukin 35 (IL35) and analogues was observed.
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
|Oncology Letters||Aberrant expression of Treg-associated cytokine IL-35 along with IL-10 and TGF-β in acute myeloid leukemia. PubMed: 22783403|
|Journal of Maternal-Fetal and Neonatal Medicine||Plasma IL-17, IL-35, interferon-γ, SOCS3 and TGF-β levels in pregnant women with preeclampsia, and their relation with severity of disease Pubmed: 24175856|
|Journal of Reproductive Immunology||What is the impact of Th1/Th2 ratio, SOCS3, IL17, and IL35 levels in unexplained infertility? Pubmed: 24368037|
|Human Immunology||The imbalanced profile and clinical significance of T helper associated cytokines in bone marrow microenvironment of the patients with acute myeloid leukemia ScienceDirect: S0198885913005776|
|Folia Biologica (Praha)||Gene Expression of Subunits of the IL-12 Family Cytokines in moDCs Derived In Vitro from the Cord Blood of Children of Healthy and Allergic Mothers Pubmed:24785110|
|Molecular Medicine Reports||Function of interleukin?17 and ?35 in the blood of patients with hepatitis B?related liver cirrhosis Pubmed:25323532|
|Rom J Morphol Embryol.||The regular distribution and expression pattern of immunosuppressive cytokine IL-35 in mouse uterus during early pregnancy Pubmed:25611266|
|Journal of Immunotherapy||Wogonin Inhibits Tumor-derived Regulatory Molecules by Suppressing STAT3 Signaling to Promote Tumor Immunity PubMed: 25962106|
|PLOS ONE||Interleukin 35 Synovial Fluid Levels Are Associated with Disease Activity of Rheumatoid Arthritis PubMed: 26204444|
|Rheumatology (Oxford)||Interleukin-35 is upregulated in systemic sclerosis and its serum levels are associated with early disease PubMed: 26231346|
|Cytokine||Anti-inflammatory effects of interleukin-35 in acquired aplastic anemia PubMed: 26282938|
|J Biol Chem||Interleukin-35 Inhibits Endothelial Cell Activation by Suppressing MAPK-AP-1 Pathway PubMed: 26085094|
|Br J Haematol||Interleukin 35 may contribute to the loss of immunological self‐tolerance in patients with primary immune thrombocytopenia PubMed: 25640666|
|Autoimmunity Reviews||New insights into immune mechanisms underlying response to Rituximab in patients with membranous nephropathy: A prospective study and a review of the literature Pubmed:26876383|
|Clinical research||Increased plasma concentrations of interleukin 35 in patients with coronary artery disease Journal:-19|
|Clinical Rheumatology||The clinical utility of serum IL-35 in patients with polymyositis and dermatomyositis pubmed:27502600|
|Respiratory Medicine||Enhanced LPS-induced activation of IL-27 signalling in sarcoidosis pubmed:27492538|
|International Journal of Molecular Medicine||Effects of an intravitreal injection of interleukin-35-expressing plasmid on pro-inflammatory andanti-inflammatory cytokines. pubmed:27460435|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|RPC008Hu01||Recombinant Interleukin 35 (IL35)||Positive Control; Immunogen; SDS-PAGE; WB.|
|APC008Hu01||Active Interleukin 35 (IL35)||Cell culture; Activity Assays.|
|PAC008Hu01||Polyclonal Antibody to Interleukin 35 (IL35)||WB; IHC; ICC; IP.|
|MAC008Hu21||Monoclonal Antibody to Interleukin 35 (IL35)||WB; IHC; ICC; IP.|
|LAC008Hu72||Biotin-Linked Monoclonal Antibody to Interleukin 35 (IL35)||WB; IHC; ICC.|
|SEC008Hu||ELISA Kit for Interleukin 35 (IL35)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|HEC008Hu||High Sensitive ELISA Kit for Interleukin 35 (IL35)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|PSC008Hu01||Antibody Pair for Interleukin 35 (IL35)||ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.|
|KSC008Hu01||ELISA Kit DIY Materials for Interleukin 35 (IL35)||Main materials for "Do It (ELISA Kit) Yourself".|