ELISA Kit DIY Materials for Brain Derived Neurotrophic Factor (BDNF)
- Product No.KSA011Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.156-10ng/mL
- Applicable Sensitivity0.064ng/mL.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- UOM 96T*596T*10 96T*20 96T*50 96T*100
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The Abs in the kit have high sensitivity and excellent specificity for detection of Brain Derived Neurotrophic Factor (BDNF).No significant cross-reactivity or interference between Brain Derived Neurotrophic Factor (BDNF) and analogues was observed.
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
|Toxicological Sciences||Interaction of PFOS and BDE-47 Co-exposure on Thyroid Hormone Levels and TH-Related Gene and Protein Expression in Developing Rat Brains PubMed: 21436126|
|Journal of the Neurological Sciences||Neuroprotective effects of adipose-derived stem cells against ischemic neuronal damage in the rabbit spinal cord ScienceDirect: S0022510X12001219|
|International Conference on Bioscience||Effect of Benthosemapterotum Peptide on Aging in Mice with Oxidative Damage Psrcentre: 212059|
|Neuroscience Letters||Chronic stress impairs learning and hippocampal cell proliferation in senescence-accelerated prone mice ScienceDirect: S0304394010015788|
|BioMed Research International||Neuroprotective effects of adipose-derived stem cells are maintained for 3 weeks against ischemic damage in the rabbit spinal cord. NCBI: PMC3925622|
|Behavioural Brain Research||The effects of Ginkgo biloba extract on cognitive functions in aged female rats: The role of oxidative stress and brain-derived neurotrophic factor Pubmed:25446810|
|Neurochemical Research||Heme Oxygenase-1 Protects Neurons from Ischemic Damage by UpreCavia (Guinea pig )lating Expression of Cu,Zn-SuperoxideDismutase, Catalase, and Brain-Derived Neurotrophic Factor in the Rabbit Spinal Cord. Pubmed:26559686|
|Exp Ther Med.||Effects of docosahexaenoic acid on learning and memory impairment induced by repeated propofol anesthesia in young rats pmc:PMC4812310|
|Genet Mol Res||Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells Pubmed:27323064|
|The International Journal af Applied Research in Veterinary Medicine||Effect of Sample Time in Clot Tube, Temperature, and Hemolysis Status on Canine Serum Brain-Derived Neurotrophic Factor (BDNF) Concentrations: Preliminary study articles:Vol14Iss3|
|J Chem Neuroanat.||Both secreted and the cellular levels of BDNF attenuated due to tau hyperphosphorylation in primary cultures of cortical neurons. pubmed:27914953|
|Int Immunopharmacol.||PPARr activation ameliorates postoperative cognitive decline probably through suppressing hippocampal neuroinflammation in aged mice. pubmed:27940378|
|The Journal of Maternal-Fetal & Neonatal Medicine||Differential expression of cord blood neurotrophins in gestational diabetes: the impact of fetal growth abnormalities pubmed:28081639|
|IOS Press Content Library||An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's … articles:journal-of-alzheimers-disease|
|Bipolar Disorders||Long‐term lithium treatment increases intracellular and extracellular brain‐derived neurotrophic factor (BDNF) in cortical and hippocampal neurons at subtherapeutic … bdi.12449|
|Elsevier||Associations of blood parameters with age, feed efficiency and sampling routine in young beef bulls science:article|
|journal of allergy and clinical immunology||Water-soluble chitosan inhibits nerve growth factor and attenuates allergic inflammation in mite allergen–induced allergic rhinitis pubmed:28412394|
|American Journal of Translational Research||A new comorbidity model and the common pathological mechanisms of migraine and epilepsy pubmed:28559979|
|European Journal of Pharmacology||Alpha-asarone attenuates depression-like behavior in nicotine-withdrawn mice: Evidence for the modulation of hippocampal pCREB levels during nicotine-withdrawal. pubmed:29042206|
|Brain, Behavior, and Immunity||An enriched environment restores hepatitis B vaccination-mediated impairments in synaptic function through IFN-γ/Arginase1 signaling Pubmed:29627530|
|Stem Cell Research & Therapy||Autophagy is required for human umbilical cord mesenchymal stem cells to improve spatial working memory in APP/PS1 transgenic mouse model Pubmed:29335016|
|Pharmacological Research||N-3 PUFA diet enrichment prevents amyloid beta-induced depressive-like phenotype Pubmed:29203442|
|Experimental Neurology||Preterm umbilical cord blood derived mesenchymal stem/stromal cells protect preterm white matter brain development against hypoxia-ischemia Pubmed:30012511|
|Nutrients||Early Life Vitamin C Deficiency Does Not Alter Morphology of Hippocampal CA1 Pyramidal Neurons or Markers of Synaptic Plasticity in a Guinea Pig Model. Pubmed:29890692|
|Neurotoxicity Research||Delta-9-Tetrahydrocannabinol (∆-THC) Induce Neurogenesis and Improve Cognitive Performances of Male Sprague Dawley Rats Pubmed:28933048|
|Neurotoxicology||Amelioration by nitric oxide (NO) mimetics on neurobehavioral and biochemical changes in experimental model of Alzheimer's disease in rats Pubmed:29522778|
|Neuroscience Letters||Long term Westernized diet leads to region-specific changes in brain signaling mechanisms Pubmed:29655945|
|Applied Physiology Nutrition and Metabolism||Changes in exercise capacity and serum BDNF following long-term sprint interval training in well-trained cyclists Pubmed: 30286300|
|Heliyon||Establishment and evaluation of a novel mouse model of peri/postmenopausal depression Doi: 10.1016/j.heliyon.2019.e01195|
|BioMed Research International||Administration of Repetitive Transcranial Magnetic Stimulation Attenuates Aβ1-42-Induced Alzheimer's Disease in Mice by Activating β-Catenin Signaling|
|International Journal of Immunopathology and Pharmacology||Effect of voluntary running on expression of myokines in brains of rats with depression Pubmed: 30834799|
|American Journal of Emergency Medicine||Clinical usefulness of brain-derived neurotrophic factor and visinin-like protein-1 in early diagnostic tests for acute stroke Pubmed: 30826210|
|Neurochemistry International||Monoaminergic system is implicated in the antidepressant-like effect of hyperoside and protocatechuic acid isolated from Impatiens glandulifera Royle in mice Pubmed: 31077758|
|REVISTA DA ASSOCIACAO MEDICA BRASILEIRA||Physical Exercise Increased Brain-Derived Neurotrophic Factor in Elderly Population with Depression Pubmed: 30994834|
|Journal of Alzheimer's Disease||Regular Exercise Enhances Cognitive Function and Intracephalic GLUT Expression in Alzheimer's Disease Model Mice Pubmed: 31561359|
|Biological Trace Element Research||Cognitive Decline of Rats with Chronic Fluorosis Is Associated with Alterations in Hippocampal Calpain Signaling Pubmed: 31797207|
|Psychopharmacology||Chronic harmine treatment has a delayed effect on mobility in control and socially defeated rats Pubmed: 32088835|
|Experimental and Therapeutic Medicine||Involvement of neurotrophic signaling in doxorubicin‑induced cardiotoxicity Pubmed: 32010279|
|Journal of Critical Care||Association of neuronal repair biomarkers with delirium among survivors of critical illness Pubmed: 31896448|
|Journal of Clinical Neuroscience||CSF levels of a set of neurotrophic factors (brain-derived neurotrophic factor, nerve growth factor) and neuropeptides (neuropeptide Y, galanin) in epileptic children Pubmed: 32327377|
|MOLECULAR NUTRITION & FOOD RESEARCH||Hyperlipidemia Down‐Regulate Brain Antioxidant Defense Enzymes and Neurotrophins in Rats: Assessment of the Modulatory Potential of EPA+ DHA and … Pubmed: 32918393|
|Neuropharmacology||Impaired LTD-like motor cortical plasticity in female patients with major depression disorder Pubmed: 32791084|
|COLLOIDS AND SURFACES B-BIOINTERFACES||Effects of gold nanoparticles administration through behavioral and oxidative parameters in animal model of parkinson's disease Pubmed: 32777662|
|Neurotoxicology||Involvement of hippocampal agmatine in β1-42 amyloid induced memory impairment, neuroinflammation and BDNF signaling disruption in mice Pubmed: 32522471|
|Journal of Veterinary Behavior||Comparison of serum brain-derived neurotrophic factor (BDNF) in dogs with and without separation anxiety|
|Food & Function||Nicotinamide riboside alleviates alcohol-induced depression-like behaviours in C57BL/6J mice by altering the intestinal microbiota associated with microglial …|
|Short-but no long-term effect of social stress in rodent depressive-like behavior is not affected by chronic treatment with Harmine|
|bioinformatics and biomedical engineering||Blood Plasma Trophic Growth Factors Predict the Outcome in Patients with Acute Ischemic Stroke|
|Overektomize dişi sıçanlarda beyin oksidatif stres, bdnf ve inflamatuar cevap düzeyleri ile nörodavranışsal değişiklikler üzerine anjiyotensin II İnhibisyonunun etkisi|
|EUROPEAN CHILD & ADOLESCENT PSYCHIATRY||Increased serum brain-derived neurotrophic factor and adrenocorticotropic hormone levels are associated with obsessive compulsive disorder in medication?free?¡ 33389158|
|BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY||Sea urchin gangliosides exhibit neuritogenic effects in neuronal PC12 cells via TrkA- and TrkB-related pathways 33589896|
|Neuropsychiatr Dis Treat||Repetitive Transcranial Magnetic Stimulation Improves Mild Cognitive Impairment Associated with Alzheimer's Disease in Mice by Modulating the miR-567 … 34239303|
|Einstein (Sao Paulo)||Tropomyosin receptor kinase B-mediated signaling in integration of neuropathic pain and obesity in diabetic polyneuropathy 34586159|
|Metab Brain Dis||Moderate-intensity continuous training and high-intensity interval training improve cognition, and BDNF levels of middle-aged overweight men 34762211|
|Transl Psychiatry||Lithium augmentation of ketamine increases insulin signaling and antidepressant-like active stress coping in a rodent model of treatment-resistant depression 34824208|
|Iranian Journal of Basic Medical Sciences||Adrenomedullin increases cAMP accumulation and BDNF expression in rat DRG and spinal motor neurons 34712429|
|J Sex Med||Endogenous Deficiency of Brain-Derived Neurotrophic Factor Induces the Downregulation of Tryptophan Hydroxylase-2 Expression in Raphe Nuclei of Rapid … 34384732|
|Cell Biochem Funct||CNTs‐CaP/chitosan‐coated AZ91D magnesium alloy extract promoted rat dorsal root ganglia neuron growth via activating ERK signalling pathway 34296452|
|J Endocrinol Invest||Serum alpha-melanocyte-stimulating hormone (a-MSH), brain-derived neurotrophic factor (BDNF), and agouti-related protein (AGRP) levels in children with Prader … Pubmed:35098494|
|Clinical Rheumatology||Gender influence on clinical manifestations, depressive symptoms and brain-derived neurotrophic factor (BDNF) serum levels in patients affected by … Pubmed:35344113|
|Sci Rep||Associations between serum levels of brain-derived neurotrophic factor, corticotropin releasing hormone and mental distress in vitiligo patients Pubmed:35508633|
|Pharmaceutics||Co-Delivery of Docosahexaenoic Acid and Brain-Derived Neurotropic Factor from Electrospun Aligned Core–Shell Fibrous Membranes in Treatment of Spinal Cord … Pubmed:35214053|
|Molecular Biology Reports||Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling … Pubmed:35585377|
|Journal of Complementary Medicine Research||Effect of herbal formulation Sharbat Ahmad Shahi on serum BDNF level in mild to moderate cases of depressive disorder|
|AFRICAN JOURNAL OF BIOCHEMISTRY RESEARCH||Medicinal effects of Saffron on obese animals by reducing Castellis risk index to protect from cardio-pathology and psychological distress|