ELISA Kit for Brain Derived Neurotrophic Factor (BDNF)
- Product No.SEA011Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range31.2-2,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 11.3pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Brain Derived Neurotrophic Factor (BDNF).
No significant cross-reactivity or interference between Brain Derived Neurotrophic Factor (BDNF) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Brain Derived Neurotrophic Factor (BDNF) and the recovery rates were calculated by comparing the measured value to the expected amount of Brain Derived Neurotrophic Factor (BDNF) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Brain Derived Neurotrophic Factor (BDNF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Brain Derived Neurotrophic Factor (BDNF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Brain Derived Neurotrophic Factor (BDNF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Toxicological Sciences||Interaction of PFOS and BDE-47 Co-exposure on Thyroid Hormone Levels and TH-Related Gene and Protein Expression in Developing Rat Brains PubMed: 21436126|
|Journal of the Neurological Sciences||Neuroprotective effects of adipose-derived stem cells against ischemic neuronal damage in the rabbit spinal cord ScienceDirect: S0022510X12001219|
|International Conference on Bioscience||Effect of Benthosemapterotum Peptide on Aging in Mice with Oxidative Damage Psrcentre: 212059|
|Neuroscience Letters||Chronic stress impairs learning and hippocampal cell proliferation in senescence-accelerated prone mice ScienceDirect: S0304394010015788|
|BioMed Research International||Neuroprotective effects of adipose-derived stem cells are maintained for 3 weeks against ischemic damage in the rabbit spinal cord. NCBI: PMC3925622|
|Behavioural Brain Research||The effects of Ginkgo biloba extract on cognitive functions in aged female rats: The role of oxidative stress and brain-derived neurotrophic factor Pubmed:25446810|
|Neurochemical Research||Heme Oxygenase-1 Protects Neurons from Ischemic Damage by UpreCavia (Guinea pig )lating Expression of Cu,Zn-SuperoxideDismutase, Catalase, and Brain-Derived Neurotrophic Factor in the Rabbit Spinal Cord. Pubmed:26559686|
|Exp Ther Med.||Effects of docosahexaenoic acid on learning and memory impairment induced by repeated propofol anesthesia in young rats pmc:PMC4812310|
|Genet Mol Res||Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells Pubmed:27323064|
|The International Journal af Applied Research in Veterinary Medicine||Effect of Sample Time in Clot Tube, Temperature, and Hemolysis Status on Canine Serum Brain-Derived Neurotrophic Factor (BDNF) Concentrations: Preliminary study articles:Vol14Iss3|
|J Chem Neuroanat.||Both secreted and the cellular levels of BDNF attenuated due to tau hyperphosphorylation in primary cultures of cortical neurons. pubmed:27914953|
|Int Immunopharmacol.||PPARr activation ameliorates postoperative cognitive decline probably through suppressing hippocampal neuroinflammation in aged mice. pubmed:27940378|
|The Journal of Maternal-Fetal & Neonatal Medicine||Differential expression of cord blood neurotrophins in gestational diabetes: the impact of fetal growth abnormalities pubmed:28081639|
|IOS Press Content Library||An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's … articles:journal-of-alzheimers-disease|
|Bipolar Disorders||Long‐term lithium treatment increases intracellular and extracellular brain‐derived neurotrophic factor (BDNF) in cortical and hippocampal neurons at subtherapeutic … bdi.12449|
|Elsevier||Associations of blood parameters with age, feed efficiency and sampling routine in young beef bulls science:article|
|journal of allergy and clinical immunology||Water-soluble chitosan inhibits nerve growth factor and attenuates allergic inflammation in mite allergen–induced allergic rhinitis pubmed:28412394|
|American Journal of Translational Research||A new comorbidity model and the common pathological mechanisms of migraine and epilepsy pubmed:28559979|
|European Journal of Pharmacology||Alpha-asarone attenuates depression-like behavior in nicotine-withdrawn mice: Evidence for the modulation of hippocampal pCREB levels during nicotine-withdrawal. pubmed:29042206|
|Brain, Behavior, and Immunity||An enriched environment restores hepatitis B vaccination-mediated impairments in synaptic function through IFN-γ/Arginase1 signaling Pubmed:29627530|
|Stem Cell Research & Therapy||Autophagy is required for human umbilical cord mesenchymal stem cells to improve spatial working memory in APP/PS1 transgenic mouse model Pubmed:29335016|
|Pharmacological Research||N-3 PUFA diet enrichment prevents amyloid beta-induced depressive-like phenotype Pubmed:29203442|
|Experimental Neurology||Preterm umbilical cord blood derived mesenchymal stem/stromal cells protect preterm white matter brain development against hypoxia-ischemia Pubmed:30012511|
|Nutrients||Early Life Vitamin C Deficiency Does Not Alter Morphology of Hippocampal CA1 Pyramidal Neurons or Markers of Synaptic Plasticity in a Guinea Pig Model. Pubmed:29890692|
|Neurotoxicity Research||Delta-9-Tetrahydrocannabinol (∆-THC) Induce Neurogenesis and Improve Cognitive Performances of Male Sprague Dawley Rats Pubmed:28933048|
|Neurotoxicology||Amelioration by nitric oxide (NO) mimetics on neurobehavioral and biochemical changes in experimental model of Alzheimer's disease in rats Pubmed:29522778|
|Neuroscience Letters||Long term Westernized diet leads to region-specific changes in brain signaling mechanisms Pubmed:29655945|