ELISA Kit DIY Materials for Matrix Metalloproteinase 10 (MMP10)

STMY2; SL2; Stromelysin 2; Transin-2

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 10 (MMP10). No significant cross-reactivity or interference between Matrix Metalloproteinase 10 (MMP10) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Journal of Cellular and Molecular Medicine Type-specific dysregulation of matrix metalloproteinases and their tissue inhibitors in end-stage heart failure patients: relationship between MMP-10 and LV remodelling Wiley: source
PLoS One. Matrix Metalloproteinase-10 Is Required for Lung Cancer Stem Cell Maintenance, Tumor Initiation and Metastatic Potential PubMed: PMC3335833
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source
Thromb Haemost. Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke Pubmed:24671655
Brain, Behavior, and Immunity Plasma inflammatory biomarkers for Huntington’s disease patients and mouse model Pubmed:25266150
Biomedical Materials In vivo bioengineered ovarian tumors based on collagen, matrigel, alginate and agarose hydrogels: a comparative study Pubmed:25634132
Brain, Behavior, and Immunity Plasma inflammatory biomarkers for Huntington’s disease patients and mouse model PubMed: 25266150
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