ELISA Kit DIY Materials for Insulin Degrading Enzyme (IDE)

Insulysin; Insulin Protease; Abeta-degrading protease; Insulinase


The Abs in the kit have high sensitivity and excellent specificity for detection of Insulin Degrading Enzyme (IDE). No significant cross-reactivity or interference between Insulin Degrading Enzyme (IDE) and analogues was observed.


1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.


Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
J Neurochem Reduction PubMed: 26442993
African Journal of Pharmacy and Pharmacology Peptide hydroxamate derivatives as regulators for insulin receptor signaling and its degradation by zinc metalloprotease in diabetic rats article-full-text-pdf
Biomedicine & Pharmacotherapy Vitamin D3 intake as regulator of insulin degrading enzyme and insulin receptor phosphorylationin diabetic rats. pubmed:27930980
Biological Trace Element Research Nigella sativa Oil and Chromium Picolinate Ameliorate Fructose-Induced Hyperinsulinemia by Enhancing Insulin Signaling and Suppressing Insulin-Degrading Enzyme in Male Rats 10.1007/s12011-017-1167-z
Neuroscience The Protective Effects of IGF-I against β-Amyloid-related Downregulation of Hippocampal Somatostatinergic System Involve Activation of Akt and Protein Kinase A Pubmed:29406271
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
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MAB897Hu22 Monoclonal Antibody to Insulin Degrading Enzyme (IDE) WB; IHC; ICC; IP.
SEB897Hu ELISA Kit for Insulin Degrading Enzyme (IDE) Enzyme-linked immunosorbent assay for Antigen Detection.
KSB897Hu01 ELISA Kit DIY Materials for Insulin Degrading Enzyme (IDE) Main materials for "Do It(ELISA Kit) Yourself"