ELISA Kit DIY Materials for Insulin Degrading Enzyme (IDE)

Insulysin; Insulin Protease; Abeta-degrading protease; Insulinase

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Insulin Degrading Enzyme (IDE). No significant cross-reactivity or interference between Insulin Degrading Enzyme (IDE) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Reference
J Neurochem Reduction PubMed: 26442993
African Journal of Pharmacy and Pharmacology Peptide hydroxamate derivatives as regulators for insulin receptor signaling and its degradation by zinc metalloprotease in diabetic rats article-full-text-pdf
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB897Hu01 Recombinant Insulin Degrading Enzyme (IDE) Positive Control; Immunogen; SDS-PAGE; WB.
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KSB897Hu01 ELISA Kit DIY Materials for Insulin Degrading Enzyme (IDE) Main materials for "Do It(ELISA Kit) Yourself"