Polyclonal Antibody to Protein Kinase C Epsilon (PKCe)

PKC-E; PRKCE; PRKC-E; NPKC-Epsilon

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against PKCe. It has been selected for its ability to recognize PKCe in immunohistochemical staining and western blotting.

USAGE

Western blotting: 1-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Magazine Reference
Am J Physiol Lung Cell Mol Physiol. Aging causes a slowing in ciliary beat frequency, mediated by PKCε. Pubmed:24487393
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