Packages (Simulation)

Polyclonal Antibody to Ferrochelatase (FECH)

Image (I)

DAB staining on IHC-P; Samples: Human Cardiac Muscle Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human FECH Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Cardiac Muscle Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human FECH Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

Figure. Western Blot; Sample: Recombinant FECH, Human.
Western Blot; Samples: Lane1: Human Liver lysate; Lane2: Porcine Heart lysate; Lane3: Hela cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human FECH Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Quality Guarantee

Instruction Manual

Polyclonal-Antibody-to-Ferrochelatase-(FECH)-PAC488Hu01.pdf

Certificate

Polyclonal Antibody to Ferrochelatase (FECH)

EPP; FCE; Protoheme Ferrolyase; Protoporphyria; Heme synthase; Protoheme ferro-lyase

Product No.PAC488Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPC488Hu01-Recombinant Ferrochelatase (FECH)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/ml
Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 105 245 US$ 245 US$ 350 US$ 875 US$ 3500
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against FECH. It has been selected for its ability to recognize FECH in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine	Citations
Free Radical Biology and Medicine	Flavonoid 4, 4′-dimethoxychalcone induced ferroptosis in cancer cells by synergistically activating Keap1/Nrf2/HMOX1 pathway and inhibiting FECH Pubmed:35697292

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPC488Hu01	Recombinant Ferrochelatase (FECH)	Positive Control; Immunogen; SDS-PAGE; WB.
PAC488Hu01	Polyclonal Antibody to Ferrochelatase (FECH)	WB; IHC; ICC; IP.
MAC488Hu22	Monoclonal Antibody to Ferrochelatase (FECH)	WB; IHC; ICC; IP.
SEC488Hu	ELISA Kit for Ferrochelatase (FECH)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMC488Hu	Multiplex Assay Kit for Ferrochelatase (FECH) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.
PGC488Hu01	Primer Pair for Ferrochelatase (FECH)	PCR