Packages (Simulation)

Polyclonal Antibody to Protein Disulfide Isomerase A4 (PDIA4)

Image (I)

Figure. FITC staining on IHC-P; Samples: Human Hela Cells.
DAB staining on IHC-P;
Samples: Human Colorectal cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human PDIA4 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Prostate cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human PDIA4 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Stomach Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human PDIA4 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Stomach cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human PDIA4 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Pancreas Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human PDIA4 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Colorectal cancer Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human PDIA4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Stomach Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human PDIA4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Pancreas Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human PDIA4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

Figure. Western Blot; Sample: Recombinant PDIA4, Human.
Western Blot; Sample: Lane1: Hela cell lysate; Lane2: Porcine Liver lysate; Lane3: Porcine Cerebrum lysate; Lane4: A431 cell lysate
Primary Ab: 3µg/ml Rabbit Anti-Human PDIA4 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification:
Lane 1: Wild-type Hela cell lysate;
Lane 2: PDIA4 knockout Hela cell lysate;
Predicted MW: 73kd
Observed MW: 73kd
Primary Ab: 3µg/ml Rabbit Anti-Human PDIA4 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Samples: Lane1: Mouse Liver lysate; Lane2: NIH/3T3 cell lysate;
Primary Ab: 0.1µg/ml Rabbit Anti-Human PDIA4 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Quality Guarantee

Instruction Manual

Polyclonal-Antibody-to-Protein-Disulfide-Isomerase-A4-(PDIA4)-PAD774Hu01.pdf

Certificate

KO-Validated

Polyclonal Antibody to Protein Disulfide Isomerase A4 (PDIA4)

PDI-A4; ERP70; ERP72; Protein Disulfide Isomerase-Associated 4; Endoplasmic reticulum resident protein 70

Product No.PAD774Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPD774Hu01-Recombinant Protein Disulfide Isomerase A4 (PDIA4)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration500µg/mL
Organism Species MoreSus scrofa; Porcine (Pig)
ApplicationsWB,IHC
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 105 245 US$ 245 US$ 350 US$ 875 US$ 3500
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against PDIA4. It has been selected for its ability to recognize PDIA4 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-3µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunofluorescence:5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
Indonesian Journal of Clinical Pathology and Medical Laboratory	Keluarga Disulfit Protein Isomerase Anggota 4(PDIA4) di Kanker Payudara dengan metastasis Unair.ac: Source
PLoS One	A novel potential biomarker for metabolic syndrome in Chinese adults: Circulating protein disulfide isomerase family A, member 4 pubmed:28650993
Acta Diabetol	Circulating protein disulfide isomerase family member 4 is associated with type 2 diabetes mellitus, insulin sensitivity, and obesity Pubmed:35460376

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPD774Hu01	Recombinant Protein Disulfide Isomerase A4 (PDIA4)	Positive Control; Immunogen; SDS-PAGE; WB.
PAD774Hu01	Polyclonal Antibody to Protein Disulfide Isomerase A4 (PDIA4)	WB,IHC
MAD774Hu21	Monoclonal Antibody to Protein Disulfide Isomerase A4 (PDIA4)	WB; IHC; ICC; IP.
SED774Hu	ELISA Kit for Protein Disulfide Isomerase A4 (PDIA4)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMD774Hu	Multiplex Assay Kit for Protein Disulfide Isomerase A4 (PDIA4) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.