CLIA Kit for Adiponectin (ADP)
GBP28; ApM1; AdipoQ; Acrp30; ACDC; APM1; C1Q And Collagen Domain Containing; Adipocyte Complement-Related Protein Of 30 KDa; Adipose Most Abundant Gene Transcript 1
- Product No.SCA605Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length2h, 40min
- Detection Range5.49-4,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 2.41pg/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Adiponectin (ADP).
No significant cross-reactivity or interference between Adiponectin (ADP) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Adiponectin (ADP) and the recovery rates were calculated by comparing the measured value to the expected amount of Adiponectin (ADP) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adiponectin (ADP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adiponectin (ADP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Adiponectin (ADP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|Substrate A||1×10mL||Substrate B||1×2mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.
|European Journal of Pharmacology||Puerarin improves insulin resistance and modulates adipokine expression in rats fed a high-fat diet PubMed: 20869961|
|BioMed Research International||Serum Fetuin-A Levels Related with Microalbuminuria in Diet-Induced Obese Rats PubMed: PMC3591142|
|PLoS One||CD40L Deficiency Attenuates Diet-Induced Adipose Tissue Inflammation by Impairing Immune Cell Accumulation and Production of Pathogenic IgG-Antibodies PubMed: PMC3297623|
|Molecular and Cellular Endocrinology||Soy isoflavones modulate adipokines and myokines to regulate lipid metabolism in adipose tissue, skeletal muscle and liver of male Huanjiang mini-pigs ScienceDirect: S0303720712004194|
|British Journal of Nutrition||n-6: n-3 PUFA ratio is involved in regulating lipid metabolism and inflammation in pigs Pubmed: 23947577|
|International Urology Nephrology||Perirenal fat associated with microalbuminuria in obese rats Pubmed: 24526332|
|Int J Mol Sci.||Maternal high folic acid supplement promotes glucose intolerance and insulin resistance in male mouse offspring fed a high-fat diet. Pubmed: 24736781|
|Mol Biol Rep.||Adiponectin decreases lipids deposition by p38 MAPK/ATF2 signaling pathway in muscle of broilers. Pubmed: 24178343|
|Endocr J.||CTRP3 modulates the expression and secretion of adipokines in 3T3-L1 adipocytes Pubmed:25168658|
|Iran J Basic Med Sci||Zataria multiflora increases insulin sensitivity and PPARγ gene expression in high fructose fed insulin resistant rats Pubmed:Pmc4046234|
|Biometals.?||Perinatal low-dose iron treatment influences susceptibility to diet-induced adipogenesis in early-aged male Wistar rats Pubmed:24519243|
|Poult Sci.?||Effect of perches on liver health of hens Pubmed:24812236|
|Gen Comp Endocrinol.||The effect of recombinant adeno-associated virus-adiponectin (rAAV2/1-Acrp30) on glycolipid dysmetabolism and liver morphology in diabetic rats Pubmed:25019654|
|Journal of Trace Elements in Medicine and Biology||Adipose tissue chromium and vanadium disbalance in high-fat fed Wistar rats Pubmed:25194956|
|J Endocrinol||Central alarin ameliorated insulin resistance of adipocytes in type 2 diabetic rats Pubmed:25240061|
|Obesity Surgery||Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats Pubmed:25697126|
|Int J Clin Exp Pathol||Cold exposure stimulates lipid metabolism, induces inflammatory response in the adipose tissue of mice and promotes the osteogenic differentiation of BMMSCs via the p38 MAPK pathway PubMed: 26617802|
|Cell Physiol Biochem||Induction of Haemeoxygenase-1 Directly Improves Endothelial Function in Isolated Aortas from Obese Rats through the Ampk-Pi3k/Akt-Enos Pathway PubMed: 26160485|
|Life Sciences||Impaired adipogenesis in adipose tissue associated with hepatic lipid deposition induced by chronic inflammation in mice with chew diet PubMed: 26188592|
|MEDICAL RESEARCH JOURNAL||Relation between afamin level and some inflammatory markers in obese children Mrjnrc: Abstract|
|Swimming training attenuates the morphological reorganization of the myocardium and local inflammation in the left ventricle of growing rats with untreated experimental diabetes. Pubmed:26896925|
|Technologia Alimentaria||Decreased adipose tissue zinc content is associated with metabolic parameters in high fat fed Wistar rats abstract:10|
|Obes Surg.||Changes on Adipose Tissue Distribution After Laparoscopic Roux-en-Y Gastric Bypass in Obese Göttingen Minipig. Effects on Glucose Metabolism. Pubmed:27185175|
|Acta Histochemica||Favorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokines science:S0065128116301234|
|Adv Biomed Res.||Trigonella foenum-graecum water extract improves insulin sensitivity and stimulates PPAR and γ gene expression in high fructose-fed insulin-resistant rats pmc:PMC4817393|
|Biological Trace Element Research||Early High-Fat Feeding Induces Alteration of Trace Element Content in Tissues of Juvenile Male Wistar Rats article:s12011-016-0777-1|
|Journal of Functional Foods||Antiobesity and antihyperlipidaemic effects of Yan-Sheng-Yin in animals and humans science:S1756464616300585|
|J Bone Miner Res||Fat and Sucrose Intake Induces Obesity‐Related Bone Metabolism Disturbances: Kinetic and Reversibility Studies in Growing and Adult Rats Pubmed:26175082|
|Journal of Molecular and Cellular Cardiology||Irisin improves perivascular adipose tissue dysfunction via regulation of the heme oxygenase-1/adiponectin axis in diet-induced obese mice pubmed:27638193|
|Journal of Diabetes Research||Central administration of galanin receptor 1 agonist boosted insulin sensitivity in adipose pubmed:27127795|
|Asian-Australasian Journal of Animal Sciences||Dietary Niacin Supplementation Suppressed Hepatic Lipid Accumulation in Rabbits pubmed:27004817|
|Biomedical and Pharmacology Journal||Association of ADIPOQ Gene Variant Rs266729 with Circulatory Adiponectin levels in Patients with type 2 Diabetes in North Indian Population: A Case- … vol10no1|
|Catalog No.||Related products for research use of Mus musculus (Mouse) Organism species||Applications (RESEARCH USE ONLY!)|
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|PAA605Mu02||Polyclonal Antibody to Adiponectin (ADP)||WB; IHC; ICC; IP.|
|PAA605Mu01||Polyclonal Antibody to Adiponectin (ADP)||WB; IHC; ICC; IP.|
|SEA605Mu||ELISA Kit for Adiponectin (ADP)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCA605Mu||CLIA Kit for Adiponectin (ADP)||Chemiluminescent immunoassay for Antigen Detection.|
|KSA605Mu01||ELISA Kit DIY Materials for Adiponectin (ADP)||Main materials for "Do It (ELISA Kit) Yourself".|