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ELISA Kit for Adiponectin (ADPN)

GBP28; ApM1; AdipoQ; Acrp30; ACDC; APM1; C1Q And Collagen Domain Containing; Adipocyte Complement-Related Protein Of 30 KDa; Adipose Most Abundant Gene Transcript 1

Specificity

This assay has high sensitivity and excellent specificity for detection of Adiponectin (ADPN).
No significant cross-reactivity or interference between Adiponectin (ADPN) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Adiponectin (ADPN) and the recovery rates were calculated by comparing the measured value to the expected amount of Adiponectin (ADPN) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 82-104 90
EDTA plasma(n=5) 78-99 84
heparin plasma(n=5) 80-103 90

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adiponectin (ADPN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adiponectin (ADPN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Adiponectin (ADPN) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 84-103% 97-105% 83-101% 93-105%
EDTA plasma(n=5) 94-102% 86-104% 98-105% 84-98%
heparin plasma(n=5) 92-105% 82-89% 78-94% 97-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
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Asian-Australasian Journal of Animal Sciences Dietary Niacin Supplementation Suppressed Hepatic Lipid Accumulation in Rabbits pubmed:27004817
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Evidence-based Complementary and Alternative Medicine Bushenkangshuai Tablet Reduces Atherosclerotic Lesion by Improving Blood Lipids Metabolism and Inhibiting Inflammatory Response via TLR4 and NF-κB Signaling … Pubmed:29619063
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Evidence-Based Complementary and Alternative Medicine BuShenKangShuai Tablet Alleviates Hepatic Steatosis via Improving Liver Adiponectin Resistance in ApoE-/-Mice
Life Sciences Interrelation of liver vascularity to non-alcoholic fatty liver through a comparative study of the vasodilator effect of carvedilol or nicorandil in rats Pubmed: 30826497
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中国药理学通报 冷暴露对高脂饮食小鼠脂肪棕色化的影响
Nutrients High-dose Glycerol Monolaurate Up-Regulated Beneficial Indigenous Microbiota without Inducing Metabolic Dysfunction and Systemic Inflammation: New Insights …
Proceedings of the Royal Society B: Biological Sciences Paternal chronic folate supplementation induced the transgenerational inheritance of acquired developmental and metabolic changes in chickens Pubmed: 31506054
Cytokine Increased plasma osteopontin levels are associated with nonalcoholic fatty liver disease in patients with type 2 diabetes mellitus Pubmed: 31514105
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Annals of Nutrition and Metabolism Serum Asprosin Concentrations Are Increased and Associated with Insulin Resistance in Children with Obesity Pubmed: 31775140
BMC Endocrine Disorders The role of the adipocytokines vaspin and visfatin in vascular endothelial function and insulin resistance in obese children Pubmed: 31771561
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Experimental and Clinical Endocrinology & Diabetes HOMA-Adiponectin Closely Associates with Cardiometabolic Risk Markers in Middle-Aged Indians with Metabolic Syndrome Pubmed: 32189318
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The Egyptian Journal of Chest Diseases and Tuberculosis Study of serum adiponectin and resistin in patients with obstructive sleep apnea
Biosciences Biotechnology Research Asia Association of+ 10211T/G (Rs17846866) Variant of Adiponectin Gene With Type 2 Diabetes Mellitus
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Scientific Reports SARS-CoV2 Induced Biochemical Mechanisms in Liver Damage and Intestinal Lesions.
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