ELISA Kit for Catenin Beta 1 (CTNNb1)
bCat; b-Cat; CTNN-B1; Cadherin-Associated Protein
- Product No.SEB021Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.156-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.057ng/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Catenin Beta 1 (CTNNb1).
No significant cross-reactivity or interference between Catenin Beta 1 (CTNNb1) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Catenin Beta 1 (CTNNb1) and the recovery rates were calculated by comparing the measured value to the expected amount of Catenin Beta 1 (CTNNb1) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Catenin Beta 1 (CTNNb1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Catenin Beta 1 (CTNNb1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Catenin Beta 1 (CTNNb1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Clinica Chimica Acta||Versican and its associated molecules: Potential diagnostic markers for multiple myeloma Pubmed:25623955|
|Journal of Bone and Mineral Research||Adverse effects of osteocytic constitutive activation of ?-catenin on bone strength and bone growth Pubmed:25639729|
|Am J Transl Res||Role of SDF-1 and Wnt signaling pathway in the myocardial fibrosis of hypertensive rats PubMed: 26396666|
|Catalog No.||Related products for research use of Rattus norvegicus (Rat) Organism species||Applications (RESEARCH USE ONLY!)|
|RPB021Ra01||Recombinant Catenin Beta 1 (CTNNb1)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAB021Ra01||Polyclonal Antibody to Catenin Beta 1 (CTNNb1)||WB; IHC; ICC; IP.|
|MAB021Ra21||Monoclonal Antibody to Catenin Beta 1 (CTNNb1)||WB; IHC; ICC; IP.|
|SEB021Ra||ELISA Kit for Catenin Beta 1 (CTNNb1)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|KSB021Ra01||ELISA Kit DIY Materials for Catenin Beta 1 (CTNNb1)||Main materials for "Do It(ELISA Kit) Yourself"|