ELISA Kit for Tumor Necrosis Factor Receptor 1 (TNFR1)
CD120A; P55; TNFRSF1A; TBP1; FPF; TNF-R; TNF-R-I; TNF-R55; TNFAR; TNFR55; TNFR60; P55-R; P60; Tumor necrosis factor receptor 1; Tumor necrosis factor-binding protein 1
- Product No.SEB499Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates and other biological fluids.
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range78.12-5000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 28pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Receptor 1 (TNFR1).
No significant cross-reactivity or interference between Tumor Necrosis Factor Receptor 1 (TNFR1) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Tumor Necrosis Factor Receptor 1 (TNFR1) and the recovery rates were calculated by comparing the measured value to the expected amount of Tumor Necrosis Factor Receptor 1 (TNFR1) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Necrosis Factor Receptor 1 (TNFR1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Necrosis Factor Receptor 1 (TNFR1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Tumor Necrosis Factor Receptor 1 (TNFR1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|PLoS ONE||A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source|
|Mediators of Inflammation||High Glucose Level Disturbs the Resveratrol-Evoked Curtailment of CX3CL1/CX3CR1 Signaling in Human Placental Circulation pubmed:28655972|
|Annals of Neurology||High complement levels in astrocyte‐derived exosomes of Alzheimer disease Pubmed:29406582|
|International Journal of Immunopathology and Pharmacology||VEGF-R2 and TNF-R1 expression and cytokine production by samples of mammary adenocarcinomas and correlations with histopathological parameters of these … Pubmed:29985074|
|Cell Death & Disease||Inhibition of transmembrane TNF-α shedding by a specific antibody protects against septic shock Pubmed: 31383857|
|Catalog No.||Related products for research use of Mus musculus (Mouse) Organism species||Applications (RESEARCH USE ONLY!)|
|RPB499Mu01||Recombinant Tumor Necrosis Factor Receptor 1 (TNFR1)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAB499Mu01||Polyclonal Antibody to Tumor Necrosis Factor Receptor 1 (TNFR1)||WB; IHC; ICC; IP.|
|LAB499Mu71||Biotin-Linked Polyclonal Antibody to Tumor Necrosis Factor Receptor 1 (TNFR1)||WB; IHC; ICC.|
|SEB499Mu||ELISA Kit for Tumor Necrosis Factor Receptor 1 (TNFR1)||Enzyme-linked immunosorbent assay for Antigen Detection.|