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Active Fibroblast Growth Factor 4 (FGF4)

HBGF4; HST; HSTF1; KFGF; K-FGF; Heparin Secretory Transforming Protein 1; Kaposi Sarcoma Oncogene; Transforming Protein KS3; FGF-Related Oncogene

ACTIVITY TEST

Fibroblast Growth Factor 4 (FGF4) is a secreted signaling protein belonging to the fibroblast growth factor family, which plays crucial roles in embryonic development, cell proliferation, differentiation, and migration. It is particularly important during limb formation, mesoderm patterning, and stem cell maintenance. FGF4 exerts its biological functions by binding and activating specific FGF receptors (FGFRs) with high affinity. FGF4 specifically binds to Fibroblast Growth Factor Receptor 2 (FGFR2), particularly the IIIc isoform, triggering receptor dimerization, autophosphorylation, and subsequent activation of downstream signaling pathways including MAPK and PI3K-AKT.To detect the activity of recombinant FGF4 , a functional ELISA assay was performed to evaluate the interaction between recombinant human FGF4 and recombinant rat FGFR2.Briefly, FGF4 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to FGFR2-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-FGF4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human FGF4 and recombinant rat FGFR2 was shown in Figure 1, the EC50 for this effect is 0.612 µg/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Molecular Neurobiology Fibroblast Growth Factor 4 Is Required but not Sufficient for the Astrocyte Dedifferentiation Springer: Source
Oncotarget Interaction between the estrogen receptor and fibroblast growth factor receptor pathways in non-small cell lung cancer pubmed:28445992
ANTICANCER RESEARCH Functional Characterization of VEGF- and FGF-induced Tumor Blood Vessel Models in Human Cancer Xenografts pubmed:29187438
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA034Hu01 Recombinant Fibroblast Growth Factor 4 (FGF4) Positive Control; Immunogen; SDS-PAGE; WB.
APA034Hu01 Active Fibroblast Growth Factor 4 (FGF4) Cell culture; Activity Assays.
PAA034Hu01 Polyclonal Antibody to Fibroblast Growth Factor 4 (FGF4) Testing in progress
MAA034Hu22 Monoclonal Antibody to Fibroblast Growth Factor 4 (FGF4) WB; IHC; ICC; IP.
SEA034Hu ELISA Kit for Fibroblast Growth Factor 4 (FGF4) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA034Hu CLIA Kit for Fibroblast Growth Factor 4 (FGF4) Chemiluminescent immunoassay for Antigen Detection.
LMA034Hu Multiplex Assay Kit for Fibroblast Growth Factor 4 (FGF4) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.