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Active Colony Stimulating Factor 3, Granulocyte (GCSF)
CSF3; G-CSF; Granulocyte Colony Stimulating Factor; Pluripoietin; Filgrastim; Lenograstim
- Product No.APA042Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder
- Purity> 90%
- Isoelectric Pointn/a
- ApplicationsCell culture; Activity Assays.
- Downloadn/a
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 317
US$ 792
US$ 1584
US$ 4752
US$ 11880
For more details, please contact local distributors!
ACTIVITY TEST
Colony Stimulating Factor 3, Granulocyte (GCSF) is a cytokine primarily produced by monocytes, macrophages, and endothelial cells. It plays a crucial role in regulating the proliferation, differentiation, and survival of neutrophils, a type of white blood cell essential for combating bacterial infections. G-CSF is widely used clinically to accelerate neutrophil recovery in patients undergoing chemotherapy or hematopoietic stem cell transplantation. It binds specifically to the Colony Stimulating Factor Receptor, Granulocyte (GCSFR), a member of the cytokine receptor family expressed on myeloid progenitor cells and mature neutrophils, thereby initiating intracellular signaling pathways that promote cell growth and function.To detect the activity of recombinant GCSF a functional ELISA assay was performed to evaluate the interaction between recombinant mouse GCSF and recombinant mouse GCSFR.Briefly, GCSF was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to GCSFR-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-GCSF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant mouse GCSF and recombinant mouse GCSFR was shown in Figure 1, the EC50 for this effect is 0.296µg/mL.
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Magazine | Citations |
| Scientific Reports | The Transcriptional Foundations of Sp110-mediated Macrophage (RAW264. 7) Resistance to Mycobacterium tuberculosis H37Ra Pubmed:26912204 |
| International Journal of Clinical and Experimental Pathology | Low-density neutrophils in severe fever with thrombocytopenia syndrome (SFTS) display decreased function to phagocytose SFTS virus and enhanced capacity to synthesize cytokines ISSN:1936-2625/IJCEP0045334 |
| International Immunopharmacology | Ginsenoside Rg3 improves cyclophosphamide-induced immunocompetence in Balb/c mice Pubmed: 30974284 |
| Mol Immunol | Oridonin alleviates kanamycin-related hearing loss by inhibiting NLRP3/caspase-1/gasdermin D-induced inflammasome activation and hair cell pyroptosis Pubmed:35749835 |