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Active Insulin Like Growth Factor 2 (IGF2)

INSIGF; IGF-II; Somatomedin A; Preptin; Insulin-like growth factor II Ala-25 Del

ACTIVITY TEST

Insulin-like Growth Factor 2 (IGF2) is a mitogenic peptide hormone critical for fetal growth and development, predominantly synthesized by the liver and placenta. It mediates signaling through the IGF1 receptor (IGF1R) and insulin receptor isoform A (IR-A) to govern cell proliferation, differentiation, and metabolic processes. Dysregulated IGF2 expression is associated with oncogenesis and overgrowth disorders such as Beckwith-Wiedemann syndrome. IGF2 exhibits high-affinity binding to Insulin-like Growth Factor Binding Protein 5 (IGFBP5), which dynamically modulates IGF2 bioactivity by either enhancing receptor engagement or restricting ligand-receptor interaction, contingent on the cellular microenvironment.Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human IGF2 and recombinant rat IGFBP5 .Briefly, biotin-linked IGF2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to IGFBP5-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of IGF2 and IGFBP5 was shown in Figure 1, the EC50 for this effect is 0.46ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
international Journal of food and nutritional sciences EVALUATION OF PHYSICO-CHEMICAL PROPERTIES OF COLOSTRUM SUPPLEMENTED DAHI Ijfans: Source
International Journal of Chemical Studies Studies on quality attributes of skimmed colostrum powder P-ISSN: 2349–8528
Neurobiology of Disease Increased motor neuron resilience by small molecule compounds that regulate IGF-II expression Pubmed:29113829
Advanced Science IGFBP2 Plays an Essential Role in Cognitive Development during Early Life Pubmed: 31832311
PLoS One Altered insulin-like growth factor-2 signaling is associated with psychopathology and cognitive deficits in patients with schizophrenia Pubmed: 32191705
Current Medical Science Changes of Serum Insulin-like Growth Factor-2 Response to Negative Symptom Improvements in Schizophrenia Patients Treated with Atypical Antipsychotics Pubmed: 32681260
ACS Omega Gastrodin Exerts Cardioprotective Action via Inhibition of Insulin-Like Growth Factor Type 2/Insulin-Like Growth Factor Type 2 Receptor Expression in Cardiac … 34250336
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
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