≡

Active Insulin Like Growth Factor Binding Protein 4 (IGFBP4)

BP4; HT29-IGFBP; IBP4

ACTIVITY TEST

Insulin-like Growth Factor Binding Protein 4 (IGFBP4) is a member of the IGFBP family, which regulates the bioavailability and activity of insulin-like growth factors (IGFs) in various tissues. IGFBP4 binds to IGF-1 and IGF-2 with high affinity, modulating their interaction with IGF receptors and thus influencing cell proliferation, differentiation, and survival. It plays critical roles in growth, development, and metabolic processes. Unlike some other IGFBPs, IGFBP4 is unique in its ability to inhibit IGF actions unless cleaved by specific proteases like PAPP-A , which releases IGFs for receptor activation.To detect the activity of recombinant mouse IGFBP4, a functional ELISA assay was performed to evaluate the interaction between recombinant mouse IGFBP1 and recombinant chicken IGF-1.Briefly, IGFBP4 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IGF-1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IGFBP4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant mouse IGFBP1 and recombinant chicken IGF-1 was shown in Figure 1, the EC50 for this effect is 0.34µg/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA055Mu01 Recombinant Insulin Like Growth Factor Binding Protein 4 (IGFBP4) Positive Control; Immunogen; SDS-PAGE; WB.
RPA055Mu02 Recombinant Insulin Like Growth Factor Binding Protein 4 (IGFBP4) Positive Control; Immunogen; SDS-PAGE; WB.
APA055Mu61 Active Insulin Like Growth Factor Binding Protein 4 (IGFBP4) Cell culture; Activity Assays.
EPA055Mu61 Eukaryotic Insulin Like Growth Factor Binding Protein 4 (IGFBP4) Positive Control; Immunogen; SDS-PAGE; WB.
PAA055Mu01 Polyclonal Antibody to Insulin Like Growth Factor Binding Protein 4 (IGFBP4) WB; IHC
PAA055Mu02 Polyclonal Antibody to Insulin Like Growth Factor Binding Protein 4 (IGFBP4) WB; IHC; ICC; IP.
SEA055Mu ELISA Kit for Insulin Like Growth Factor Binding Protein 4 (IGFBP4) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA055Mu Multiplex Assay Kit for Insulin Like Growth Factor Binding Protein 4 (IGFBP4) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA055Mu01 ELISA Kit DIY Materials for Insulin Like Growth Factor Binding Protein 4 (IGFBP4) Main materials for "Do It (ELISA Kit) Yourself".