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Active Interleukin 15 (IL15)

ACTIVITY TEST

Interleukin 15 (IL15) is a widely expressed cytokine that is structurally and functionally related to IL2, which plays an important role in many immunological diseases. IL15 also regulates T and natural killer (NK) cell activation and proliferation. The activity of recombinant pig IL15 was measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. MO7e cells were seeded into triplicate wells of 96-well plates at a density of 30,000 cells/well in RPMI-1640 with the addition of various concentrations of rpIL15. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 °C. Cell proliferation of MO7e cells after incubation with rpIL15 for 72h observed by inverted microscope was shown in Figure 1. The dose-effect curve of rpIL15 was shown in Figure 2. It was obvious that rpIL15 significantly promoted cell proliferation of MO7e cells .The EC50 for this effect is typically 0.55 ug/ml.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Endocrine Expression of interleukin-15 and inflammatory cytokines in skeletal muscles of STZ-induced diabetic rats: effect of resistance exercise training Pubmed: 24006180
Endocrine Time course of IL-15 expression after acute resistance exercise in trained rats: effect of diabetes and skeletal muscle phenotype Pubmed:25522723
Med Sci Moni miR-15b is Downregulated in Myasthenia Gravis Patients and Directly Regulates the Expression of Interleukin-15 (IL-15) in Experimental Myasthenia Gravis Mice PubMed: 26087886
Exercise physiology Effects of intrinsic aerobic capacity, aging and physical activity on interleukin-15 protein level in serum and skeletal muscle Dspace: Handle
Biotechnology Letters Novel adjuvant for immunization against tuberculosis: DNA vaccine expressing Mycobacterium tuberculosis antigen 85A and interleukin-15 fusion product elicits strong immune responses in mice s10529-017-2342-1
Journal of Controlled Release Interleukin-15 and cisplatin co-encapsulated thermosensitive polypeptide hydrogels for combined immuno-chemotherapy. pubmed:28408199
Catalog No. Related products for research use of Sus scrofa; Porcine (Pig) Organism species Applications (RESEARCH USE ONLY!)
RPA061Po01 Recombinant Interleukin 15 (IL15) Positive Control; Immunogen; SDS-PAGE; WB.
APA061Po01 Active Interleukin 15 (IL15) Cell culture; Activity Assays.
PAA061Po01 Polyclonal Antibody to Interleukin 15 (IL15) WB; IHC; ICC; IP.
CGA061Po01 cDNA of Interleukin 15 (IL15) PCR; Protein Expression; etc.