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Active Oncostatin M (OSM)

OS-M

ACTIVITY TEST

Oncostatin M (OSM), encoded by the OSM gene, is a pleiotropic cytokine belonging to the interleukin-6 (IL-6) family. Initially identified for its anti-tumor activity, it is primarily secreted by activated T cells, macrophages, and dendritic cells. OSM plays pivotal roles in regulating cell growth, differentiation, inflammation, and tissue remodeling, with implications in immune responses, embryonic development, and pathological processes like fibrosis and autoimmune diseases. Its biological effects are mediated through binding to specific receptor complexes on target cell surfaces. OSM and LIF (Leukemia Inhibitory Factor) share structural homology and can bind to the common gp130 receptor subunit, enabling functional crosstalk in cellular signaling.Thus a functional ELISA assay was conducted to detect the interaction of recombinant mouse OSM and recombinant rat LIF.
Briefly, OSM was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to LIF-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-OSM pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant mouse OSM and recombinant rat LIF was shown in Figure 1, the EC50 for this effect is 0.322µg/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Journal of Obstetrics and Gynaecology Research Oncostatin M as a target biological molecule of preeclampsia PubMed: 20149034
J Neuroinflammation Oncostatin M promotes excitotoxicity by inhibiting glutamate uptake in astrocytes: implications in HIV-associated neurotoxicity via Smad2/4 in Human Renal Proximal Tubular Epithelial Cells Pubmed:27287400
22 Modulating the phenotype of host macrophages to enhance osteogenesis in MSC-laden hydrogels: Design of a glucomannan coating material. pubmed:28582717
Advanced Functional Materials Fungal Component Coating Enhances Titanium Implant‐Bone Integration Doi: 10.1002/adfm.201804483
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA110Mu01 Recombinant Oncostatin M (OSM) Positive Control; Immunogen; SDS-PAGE; WB.
RPA110Mu02 Recombinant Oncostatin M (OSM) Positive Control; Immunogen; SDS-PAGE; WB.
EPA110Mu61 Eukaryotic Oncostatin M (OSM) Positive Control; Immunogen; SDS-PAGE; WB.
APA110Mu01 Active Oncostatin M (OSM) Cell culture; Activity Assays.
PAA110Mu01 Polyclonal Antibody to Oncostatin M (OSM) WB
PAA110Mu02 Polyclonal Antibody to Oncostatin M (OSM) IHC
SEA110Mu ELISA Kit for Oncostatin M (OSM) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA110Mu Multiplex Assay Kit for Oncostatin M (OSM) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.