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Active Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL)

CD253; TNFSF10; APO2L; Apo2-L; TL2; TRAIL; Tumor Necrosis Factor Ligand Superfamily Member 10; Apo-2 Ligand

ACTIVITY TEST

Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL), also designated TNFSF10, is a type II transmembrane protein belonging to the TNF superfamily. It functions as a key cytokine that selectively triggers extrinsic apoptosis in cancer cells while sparing most normal cells, making it a promising candidate for anticancer therapy. TRAIL exists as a homotrimer and binds to death receptors DR4 and DR5 to activate caspase‑mediated apoptotic signaling. It is widely expressed in immune cells and various tissues, participating in immune surveillance and tumor suppression. Unlike many TNF family members, TRAIL exhibits minimal systemic toxicity due to decoy receptors in normal tissues that block excessive signaling.TRAIL does not directly bind FAS; they share parallel apoptotic pathways via distinct death receptors.To detect the activity of recombinant TRAIL , a functional ELISA assay was performed to evaluate the interaction between recombinant dog TRAIL and recombinant human FAS. Briefly, TRAIL was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to FAS-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TRAIL pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant dog TRAIL and recombinant human FAS was shown in Figure 1, the EC50 for this effect is 0.012 µg/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
PLOS ONE Intranasal Administration of Recombinant TRAIL Down-Regulates CXCL-1/KC in an Ovalbumin-Induced Airway Inflammation Murine Model Pubmed:25506835
PLOS ONE Soluble TRAIL Concentration in Serum Is Elevated in People with Hypercholesterolemia PubMed: 26633016
Scientific Reports Non-alcoholic fatty liver disease, vascular inflammation and insulin resistance are exacerbated by TRAIL deletion in mice pubmed:28507343
Journal of Molecular Medicine TRAIL and TRAIL Receptors as Prognostic Markers in Breast Cancer Patients Pubmed: 31183506
life sciences Nifuroxazide attenuates experimentally-induced hepatic encephalopathy and the associated hyperammonemia and cJNk/caspase-8/TRAIL activation in rats Pubmed: 32259601
Int Immunopharmacol Cardio-protective impact of gabapentin against doxorubicin-induced myocardial toxicity in rats; emphasis on modulation of inflammatory-apoptotic signaling Pubmed: 33199237
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY Chemo-preventive effect of crocin against experimentally-induced hepatocarcinogenesis via regulation of apoptotic and Nrf2 signaling pathways Pubmed: 32942000
J Biochem Mol Toxicol Tranilast abrogates cisplatin©\induced testicular and epididymal injuries: An insight into its modulatory impact on apoptosis/proliferation 34047436
Catalog No. Related products for research use of Canis familiaris; Canine (Dog) Organism species Applications (RESEARCH USE ONLY!)
RPA139Ca01 Recombinant Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) Positive Control; Immunogen; SDS-PAGE; WB.
APA139Ca01 Active Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) Cell culture; Activity Assays.
PAA139Ca01 Polyclonal Antibody to Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) WB
MAA139Ca21 Monoclonal Antibody to Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) WB; IHC; ICC; IP.
SEA139Ca ELISA Kit for Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA139Ca Multiplex Assay Kit for Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.