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Active Vascular Endothelial Growth Factor C (VEGFC)

VEGF-C; Flt4-L; VRPL; Vascular Endothelial Growth Factor-Related Protein

ACTIVITY TEST

Vascular Endothelial Growth Factor C (VEGFC) is a key lymphangiogenic factor that primarily binds to VEGFR-3 (FLT4), promoting lymphatic endothelial cell proliferation, migration, and survival. It also activates VEGFR-2 (KDR) upon proteolytic processing, contributing to angiogenesis. VEGFC is essential for embryonic lymphatic development and adult lymphatic remodeling.Besides,Vascular Endothelial Growth Factor D (VEGFD) has been identified as an interactor of VEGFC, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant rabbit VEGFC and recombinant bovine VEGFD . Briefly, biotin-linked VEGFC were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to VEGFD-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of recombinant rabbit VEGFC and recombinant bovine VEGFD was shown in Figure 1, the EC50 for this effect is 0.43ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Journal of Cellular Biochemistry Alternatively activated RAW264. 7 macrophages enhance tumor lymphangiogenesis in mouse lung adenocarcinoma PubMed: 19241443
genetics and molecular research Expression of COX-2 and VEGF-C in cholangiocarcinomas at different clinical and pathological stages PubMed: 26125824
Experimental and Therapeutic Fenofibrate inhibits the expression of VEGFC and VEGFR-3 in retinal pigmental epithelial cells exposed to hypoxia PubMed: 26622498
Am J Pathol IL-10 Indirectly Regulates Corneal Lymphangiogenesis and Resolution of Inflammation via Macrophages PubMed: 26608451
PLoS One. Interleukin-6 Induces Vascular Endothelial Growth Factor-C Expression via Src-FAK-STAT3 Signaling in Lymphatic Endothelial Cells. pubmed:27383632
Oncology Letters RNAi-mediated gene silencing of vascular endothelial growth factor C suppresses growth andinduces apoptosis in mouse breast cancer in vitro and in vivo. pubmed:27895746
Angiogenesis Characterization of isolated liver sinusoidal endothelial cells for liver bioengineering Pubmed:29582235
Oncology Reports Effects of diphyllin as a novel V-ATPase inhibitor on TE-1 and ECA-109 cells Pubmed:29328465
LABORATORY INVESTIGATION Dynamic signature of lymphangiogenesis during acute kidney injury and chronic kidney disease Pubmed: 31019289
Cutaneous and Ocular Toxicology Isotretinoin does not alter VEGF-A and VEGF-C levels: Do retinoids behave differently in dose-dependent and/or in vivo/in vitro conditions? Pubmed: 32722957
Catalog No. Related products for research use of Oryctolagus cuniculus (Rabbit) Organism species Applications (RESEARCH USE ONLY!)
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