≡

Active Meprin A Beta (MEP1b)

PPH Beta; Endopeptidase-2; Meprin B; PABA Peptide Hydrolase; N-benzoyl-L-tyrosyl-P-amino-Benzoic Acid Hydrolase Subunit Beta

ACTIVITY TEST

Meprin A Beta, also known as MEP1B, is a zinc-dependent metalloprotease that belongs to the astacin family. This protease can form oligomeric complexes, such as with Meprin A Alpha, and is predominantly expressed in the kidney, intestine, and immune cells. Meprin A Beta plays a crucial role in extracellular matrix remodeling, inflammatory responses, and cell adhesion by cleaving various substrates, including collagen, cytokines like IL-1β and IL-6, and adhesion molecules. It exists in both membrane-anchored and soluble forms.Besides,MMP9 has been identified as an interactor of MEP1B, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human MEP1B and recombinant bovine MMP9. Briefly, Matrix Metalloproteinase 9 (MMP9) was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to MMP9-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-MEP1B pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human MEP1B and recombinant bovine MMP9 was shown in Figure 1, the EC50 for this effect is 0.029 µg/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APA173Hu01 Active Meprin A Beta (MEP1b) Cell culture; Activity Assays.
RPA173Hu01 Recombinant Meprin A Beta (MEP1b) Positive Control; Immunogen; SDS-PAGE; WB.
PAA173Hu01 Polyclonal Antibody to Meprin A Beta (MEP1b) WB
MAA173Hu21 Monoclonal Antibody to Meprin A Beta (MEP1b) WB; IHC; ICC; IP.
SEA173Hu ELISA Kit for Meprin A Beta (MEP1b) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA173Hu ELISA Kit for Meprin A Beta (MEP1b) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA173Hu Multiplex Assay Kit for Meprin A Beta (MEP1b) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.