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Active Platelet/Endothelial Cell Adhesion Molecule (PECAM1)

CD31; EndoCAM; GPIIA'; PECA1

ACTIVITY TEST

Platelet/Endothelial Cell Adhesion Molecule (PECAM1), also known as CD31, is a transmembrane glycoprotein expressed on endothelial cells, platelets, and leukocytes. It has six immunoglobulin - like domains.PECAM1 maintains vascular integrity via homophilic binding, regulating endothelial cell migration during angiogenesis. It also modulates immune responses by influencing leukocyte movement across the endothelium.Notably, its interaction with Integrin Beta 2 (CD18) promotes leukocyte - endothelial adhesion and transmigration, key for immune cell recruitment to inflammation sites.Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant rat PECAM1 and recombinant human CD18 . Briefly, biotin-linked PECAM1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to CD18-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of PECAM1 and CD18 was shown in Figure 1,and this effect was in a dose dependent manner.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
BioMed research international Endothelial gene expression and molecular changes in response to radiosurgery in in vitro and in vivo models of cerebral arteriovenous malformations Pubmed: 24199192
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