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Active Gelsolin (GSN)

GS; AGEL; ADF; Brevin; Gelsolin Amyloidosis,Finnish Type; Actin-depolymerizing factor

ACTIVITY TEST

Gelsolin (GSN) is a ubiquitous, multifunctional actin-binding protein that plays a pivotal role in remodeling the cytoskeleton by severing, capping, and nucleating actin filaments. This calcium-dependent regulation is essential for critical cellular processes including cell motility, shape changes, and the clearance of cellular debris post-injury. By controlling the dynamics of actin assembly and disassembly, GSN influences a wide range of physiological functions from wound healing to platelet activation. Importantly, GSN directly binds to ACTC1, the primary actin isoform in cardiac muscle, to regulate the contractile apparatus and maintain heart function.To detect the activity of recombinant GSN , a functional ELISA assay was performed to evaluate the interaction between recombinant mouse GSN and recombinant human ACTC1.Briefly, GSN was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to ACTC1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-GSN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant mouse GSN and recombinant human ACTC1 was shown in Figure 1, the EC50 for this effect is 0.38µg/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Neurochmical Research Gelsolin in Cerebrospinal Fluid as a Potential Biomarker of Epilepsy SpringerLink: 3585r8h46q7r0171
Journal of Neuroinflammation Treatment with gelsolin reduces brain inflammation and apoptotic signaling in mice following thermal injury BioMed: 17422094
Biomedical Engineering and Informatics Application of iTRAQ quantitative proteomics in identification of serum biomarkers in breast cancer IEEE: 6098563
European Journal of Obstetrics & Gynecology and Reproductive Biology Investigation of the actin scavenging system in pre-eclampsia Pubmed: 24239294
The Annals of Thoracic Surgery Proteomic Study Reveals Plasma Protein Changes in Congenital Heart Diseases Pubmed: 24565402
BMC Anesthesiol.? The role of plasma gelsolin in cardiopulmonary bypass induced acute lung injury in infants and young children: a pilot study Pubmed:Pmc4132929
Cell Biochemistry and Biophysics Gelsolin: Role of a Functional Protein in Mitigating Radiation Injury Pubmed:25164111
Neurosci Lett Plasma gelsolin and matrix metalloproteinase 3 as potential biomarkers for Alzheimer disease PubMed: 25864780
Proteomics Clinical Applications Comparative mass spectrometric and immunoassay‐based proteome analysis in serum of Duchenne muscular dystrophy patients Pubmed:26680509
PROTEOMICS - Clinical Applications Comparative mass spectrometric and immunoassay-based proteome analysis in serum ofDuchenne muscular dystrophy patients. pubmed:26680509
Medicina Intensiva Serum gelsolin levels in aneurismal subarachnoid hemorrhage: Preliminary results. pubmed:28365030
Journal of Alzheimers Disease Urine-Based Biomarkers for Alzheimer's Disease Identified Through Coupling Computational and Experimental Methods Pubmed:30040720
World Journal of Pediatrics Plasma gelsolin level predicts acute kidney injury after cardiopulmonary bypass in infants and young children Pubmed:29427164
Biomedicines Production and Secretion of Gelsolin by Both Human Macrophage-and Fibroblast-like Synoviocytes and GSN Modulation in the Synovial Fluid of Patients with Various … Pubmed:35327525
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
APA372Mu01 Active Gelsolin (GSN) Cell culture; Activity Assays.
RPA372Mu01 Recombinant Gelsolin (GSN) Positive Control; Immunogen; SDS-PAGE; WB.
RPA372Mu02 Recombinant Gelsolin (GSN) Positive Control; Immunogen; SDS-PAGE; WB.
PAA372Mu02 Polyclonal Antibody to Gelsolin (GSN) IHC
PAA372Mu01 Polyclonal Antibody to Gelsolin (GSN) WB; IHC
SEA372Mu ELISA Kit for Gelsolin (GSN) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA372Mu Multiplex Assay Kit for Gelsolin (GSN) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.