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Active Early Growth Response Protein 1 (EGR1)

Zif268; AT225; G0S30; KROX24; NGFI-A; TIS8; ZIF-268; ZNF225; NGFIA; TIS8; ZENK; Nerve Growth Factor-Induced Protein A; Transcription Factor ETR103; Zinc Finger Protein 225

ACTIVITY TEST

Early Growth Response Protein 1 (EGR1) is a zinc-finger transcription factor that plays a pivotal role in various cellular processes. It is rapidly and transiently induced in response to a wide range of extracellular stimuli, including growth factors, cytokines, oxidative stress, and neuronal activity. This immediate early gene product acts as a molecular switch, regulating the expression of downstream target genes involved in cell proliferation, differentiation, apoptosis, and synaptic plasticity. To test the effect of EGR1 on cell proliferation, SKOV3 cells were seeded into triplicate wells of 96-well plates and allowed to attach, replaced with various concentrations of recombinant mouse EGR1. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 ℃. Cell viability was assessed by CCK-8 assay after incubation with recombinant mouse EGR1 for 72h. The result was shown in Figure 1. It was obvious that EGR1 significantly decreased cell viability of SKOV3 cells. The ED50 of recombinant mouse EGR1 is 2.836 μg/ml.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Magazine Citations
The Journal of Biological Chemistry Gekko Sulfated Glycopeptide Inhibits Tumor Angiogenesis by Targeting Basic Fibroblast Growth Factor Jcb: 321521
Oncology Letters Expression of early growth response gene-1 in precancerous lesions of gastric cancer. pubmed:27698846
Investigative Ophthalmology & Visual Science Gene Signature-Based Development of ELISA Assays for Reproducible Qualification of CulturedHuman Corneal Endothelial Cells. pubmed:27552407
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA416Mu02 Recombinant Early Growth Response Protein 1 (EGR1) Positive Control; Immunogen; SDS-PAGE; WB.
RPA416Mu01 Recombinant Early Growth Response Protein 1 (EGR1) Positive Control; Immunogen; SDS-PAGE; WB.
APA416Mu01 Active Early Growth Response Protein 1 (EGR1) Cell culture; Activity Assays.
PAA416Mu02 Polyclonal Antibody to Early Growth Response Protein 1 (EGR1) WB; IHC; ICC; IP.
PAA416Mu01 Polyclonal Antibody to Early Growth Response Protein 1 (EGR1) WB
SEA416Mu ELISA Kit for Early Growth Response Protein 1 (EGR1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA416Mu Multiplex Assay Kit for Early Growth Response Protein 1 (EGR1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.