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Active Immunoglobulin E (IgE)

IGHE; Immunoglobulin Heavy Constant Epsilon; Ig epsilon chain C region

ACTIVITY TEST

Immunoglobulin E (IgE) is a key class of antibody central to type I hypersensitivity and antiparasitic immunity. Produced mainly by plasma cells in mucosal tissues, IgE is present at very low concentrations in serum compared to other immunoglobulins. It binds with high affinity to FcεRI on mast cells and basophils, sensitizing these cells for allergic reactions upon allergen exposure. Elevated IgE levels are closely linked to allergic diseases such as asthma, rhinitis, and atopic dermatitis. Beyond allergy, IgE contributes to immune surveillance against parasites and modulates immune cell functions. IgE binds CD23 (FcεRII) via its Cε3–Cε4 domains in a low‑affinity, calcium‑dependent interaction.Thus a functional ELISA assay was conducted to detect the interaction of recombinant human IgE and mouse recombinant CD23. Briefly, IgE was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CD23-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IgE pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human IgE and recombinant mouse CD23 was shown in Figure 1, the EC50 for this effect is 0.1039µg/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
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Ann Allergy Asthma Immunol Reduced airway microbiota diversity is associated with elevated allergic respiratory inflammation PubMed: 26123423
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Gut Pathog. Oral administration of Clostridium butyricum CGMCC0313‐1 reduces ovalbumin‐induced allergic airway inflammation in mice pubmed:28250847
International Immunopharmacology Catalpol alleviates ovalbumin-induced asthma in mice: Reduced eosinophil infiltration in the lung pubmed:27992791
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Molecular Medicine Reports Protective effects of astragaloside IV against ovalbumin-induced allergic rhinitis are mediated by T-box protein expressed in T cells/GATA-3 and forkhead box protein 3/retinoic acid-related orphan nuclear receptor γt. pubmed:28586019
Journal of Autoimmunity Commensal bacteria aggravate allergic asthma via NLRP3/IL-1β signaling in post-weaning mice 10.1016:j.jaut.2018.07.003
Water Research  Sub-chronic inhalation of reclaimed water-induced fibrotic lesion in a mouse model Pubmed:29655095
Human & Experimental Toxicology Investigation of hypersensitivity potential of diacetyl by determining cytokine profiles Pubmed:29233007
Journal of International Medical Research Changes in Th1/Th2-producing cytokines during acute exacerbation chronic obstructive pulmonary disease Pubmed:29950127
Journal Of Cellular Biochemistry Effects of catalpol on bronchial asthma and its relationship with cytokines Pubmed: 30536454
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES  Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat Model Pubmed: 30781605
Royal Society Open Science Metabolomics analysis of baicalin on ovalbumin-sensitized allergic rhinitis rats
Health and Medicine Antiallergical Effect of New Combined Nazal Aerodisperse System in the Conditions of Experimental Allergic Rhinitis
American Journal of Translational Research Inhibitory effects of catalpol coordinated with budesonide and their relationship with cytokines and Interleukin-13 expression Pubmed: 31737193
MOLECULAR IMMUNOLOGY Animal models for analysis of hypersensitivity reactions to Shuanghuanglian injection Pubmed: 32302806
bioscience reports Protective effect of Asarum sieboldii essential oil on ovalbumin induced allergic rhinitis in rat Pubmed: 32395767
international journal of antimicrobial agents Synergistic Phage-surfactant Combination Clears IgE-promoted Staphylococcus aureus Aggregation in Vitro and Enhances the Effect in Vivo Pubmed: 32335278
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Biomarkers of Allergic Asthma and their Association with Serum Parameters
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Expert Opinion on Therapeutic Targets Dapagliflozin mitigates ovalbumin-prompted airway inflammatory-oxidative successions and associated bronchospasm in a rat model of allergic asthma Pubmed:35549595
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