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Active Thrombospondin 1 (THBS1)

TSP1; Thrombospondin-1p180

ACTIVITY TEST

Thrombospondin 1 (THBS1) is a matricellular glycoprotein that critically mediates cell–matrix interactions. It promotes cell adhesion by binding to cell surface receptors, including integrins such as αvβ3 and α4β1, and non-integrin receptors like CD36 and CD47. Through its multiple domains, THBS1 forms a molecular bridge between the extracellular matrix and the cell membrane, facilitating focal adhesion assembly and cytoskeletal organization. This adhesive function is essential for modulating cellular processes such as migration, proliferation, and survival, positioning THBS1 as a key regulator in tissue remodeling, wound healing, and angiogenesis. As THBS1 has the function of cell adhesion, we measure the activity of recombinant mouse THBS1 by the ability of the immobilized protein to support the adhesion of AGS cell. When cells are added to different concentrations of recombinant mouse THBS1 coated plates, cells will adhere after 2 hour incubation at 37 ℃. The adhesion of AGS after 2 hour incubation at 37 ℃ observed by inverted microscope was shown in Figure 1. Cell adherent was in a dose dependent manner, the result was shown in Figure 2, the EC50 was 0.6 μg/ml.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Journal of Physiology and Biochemistry Glucose and insulin modify thrombospondin 1 expression and secretion in primary adipocytes from diet-induced obese rats SpringerLink: 1v552w21p7423764
Basic Research in Cardiology Secretome of apoptotic peripheral blood cells (APOSEC) attenuates microvascular obstruction in a porcine closed chest reperfused acute myocardial infarction model: role of platelet aggregation and vasodilation PubMed: 22899170
PloS one Anthocyanin-Rich Purple Corn Extract Inhibit Diabetes-Associated Glomerular Angiogenesis Pubmed: 24278186
BMC Complementary and Alternative Medicine Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical study Pubmed:25652121
Oncotarget Novel circulating peptide biomarkers for esophageal squamous cell carcinoma revealed by a magnetic bead-based MALDI-TOFMS assay pubmed:26993605
Osteoarthritis and Cartilage Orosomucoid 2 serves for predicting but not for monitoring drug response in patients with knee osteoarthritis treated with chondroitin sulfate/glucosamine … 10.1016:j.joca.2018.02.412
GEBURTSHILFE UND FRAUENHEILKUNDE Prediction of Spontaneous Preterm Birth in At-risk Women Using Thrombospondin 1 from Cervicovaginal Fluid: A Prospective Observational Study 34531612
NPJ Regen Med Mesenchymal stromal cells mitigate liver damage after extended resection in the pig by modulating thrombospondin-1/TGF-β 34862411
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA611Mu02 Recombinant Thrombospondin 1 (THBS1) Positive Control; Immunogen; SDS-PAGE; WB.
RPA611Mu01 Recombinant Thrombospondin 1 (THBS1) Positive Control; Immunogen; SDS-PAGE; WB.
RPA611Mu03 Recombinant Thrombospondin 1 (THBS1) Positive Control; Immunogen; SDS-PAGE; WB.
APA611Mu01 Active Thrombospondin 1 (THBS1) Cell culture; Activity Assays.
PAA611Mu02 Polyclonal Antibody to Thrombospondin 1 (THBS1) WB; IHC
PAA611Mu01 Polyclonal Antibody to Thrombospondin 1 (THBS1) WB
PAA611Mu03 Polyclonal Antibody to Thrombospondin 1 (THBS1) WB
LAA611Mu71 Biotin-Linked Polyclonal Antibody to Thrombospondin 1 (THBS1) WB
WEA611Mu Wide-range ELISA Kit for Thrombospondin 1 (THBS1) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA611Mu High Sensitive ELISA Kit for Thrombospondin 1 (THBS1) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA611Mu ELISA Kit for Thrombospondin 1 (THBS1) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA611Mu CLIA Kit for Thrombospondin 1 (THBS1) Chemiluminescent immunoassay for Antigen Detection.
LMA611Mu Multiplex Assay Kit for Thrombospondin 1 (THBS1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.