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Active CCAAT/Enhancer Binding Protein Gamma (CEBPg)

CEBP-G; GPE1BP; IG/EBP-1

ACTIVITY TEST

CCAAT/Enhancer Binding Protein Gamma (CEBPg) is a transcription factor belonging to the bZIP family, widely expressed across human tissues. It plays a crucial role in regulating immune and inflammatory responses by binding to C/EBP regulatory elements in target gene promoters. CEBPg modulates granulopoiesis, acute-phase reactions, and cytokine production, often acting redundantly or synergistically with other C/EBP family members. Dysregulation of CEBPg is linked to autoimmune diseases and hematological malignancies, highlighting its importance in maintaining immune homeostasis.Human CEBPg forms heterodimers with human ATF4 to regulate stress-responsive gene expression.Briefly, biotin-linked CEBPg were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100µl were then transferred to ATF4-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of human CEBPg and human ATF4 was shown in Figure 1, the EC50 for this effect is 0.271µg/mL.

USAGE

Reconstitute in ddH2O to a concentration of 0.5-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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