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Active Protein Kinase, AMP Activated Alpha 1 (AMPK Alpha 1)

PRKAa1; AMPK; AMPKa1; PKA; ACACA kinase; 5'-AMP-Activated Protein Kinase Catalytic Subunit Alpha-1; Acetyl-CoA carboxylase kinase; Hydroxymethylglutaryl-CoA reductase kinase

ACTIVITY TEST

AMPK alpha 1, also known as AMP-activated protein kinase alpha 1 subunit, is a crucial enzyme that plays a significant role in energy metabolism. It is is involved in regulating several metabolic processes, including glucose and fatty acid uptake, oxidation, and storage. It promotes energy-producing pathways, such as glycolysis, fatty acid oxidation, and mitochondrial biogenesis, while inhibiting energy-consuming processes, like protein synthesis and lipogenesis. Besides, PRKAb1 has been identified as an interactor of AMPK Alpha 1, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human AMPK Alpha 1 and recombinant human PRKAb1. Briefly, AMPK Alpha 1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to PRKAb1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-AMPK Alpha 1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human AMPK Alpha 1 and recombinant human PRKAb1 was shown in Figure 1, the EC50 for this effect is 0.94 ug/mL.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Scientific Reports Deletion of the serine protease CAP2/Tmprss4 leads to dysregulated renal water handling upon dietary potassium depletion Pubmed: 31863073
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