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Active Lactoferrin (LTF)

HLF2; LF; GIG12; Lactotransferrin; Growth-inhibiting protein 12; Talalactoferrin; Lfcin-H; Kaliocin-1

ACTIVITY TEST

Lactoferrin (LTF), a member of the transferrin family of iron-binding glycoproteins, is a multifunctional protein predominantly secreted by exocrine glands and immune cells. It exerts potent iron-sequestering activity to deprive pathogens of essential iron, mediating broad-spectrum antimicrobial effects. Beyond innate immunity, LTF exhibits robust antiproliferative and proapoptotic properties in malignant cells: it modulates cell cycle checkpoints to arrest proliferation and activates caspase-dependent apoptotic signaling pathways, inducing programmed cell death in cancer cells. Additionally, LTF regulates immune responses and anti-inflammatory processes, making it a promising candidate for anticancer and antimicrobial therapeutic development. To test the effect of LTF on cell apoptosis, HCT116 cells were seeded into triplicate wells of 96-well plates at a density of 4,000 cells/well and allowed to attach overnight, then the medium was replaced with various concentrations of recombinant bovine LTF diluted with 5% serum standard DMEM. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 ℃. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant bovine LTF for 72h. The result was shown in Figure 1. It was obvious that LTF significantly decreased cell viability of HCT116 cells. The ED50 of recombinant bovine LTF is 2.91 μg/ml.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Magazine Citations
The International Journal of Biochemistry & Cell Biology Changes of hepatic lactoferrin gene expression in two mouse models of the acute phase reaction ScienceDirect: S1357272511002524
BMC Veterinary Research Changes in composition of colostrum of Egyptian buffaloes and Holstein cows BioMed: 17466148
Plos One p38MAPK, ERK and PI3K Signaling Pathways Are Involved in C5a-Primed Neutrophils for ANCA-Mediated Activation PlosOne: source
The Journal of Biological Chemistry Sphingolipid Pathway Regulates Innate Immune Responses at the Fetomaternal Interface during Pregnancy Pubmed:25505239
PLoS One Control of Methicillin-Resistant Staphylococcus aureus Pneumonia Utilizing TLR2 Agonist Pam3CSK4 Pubmed:26974438
Bioscience, Biotechnology, and Biochemistry Salivary lactoferrin is transferred into the brain via the sublingual route pubmed:28351211
Pulmonary Pharmacology & Therapeutics Effects of fluticasone propionate and budesonide on the expression of immune defense genes in bronchial epithelial cells Pubmed:29627483
Microbial Pathogenesis TLR2 agonist Pam3CSK4 enhances the antibacterial functions of GM-CSF induced neutrophils to methicillin-resistant Staphylococcus aureus
Assessment of the Diagnostic Value of IL-10 and Lactoferrin by BIRADS Categories in Breast Neoplasms
J Clin Med A Three-Protein Panel to Support the Diagnosis of Sepsis in Children Pubmed:35329889
Food Funct Three Urban of China: A Cohort Study of Maternal-Infant Factors and HM Protein Components Pubmed:35438093
Biomedicines Myeloid-Specific Pyruvate-Kinase-Type-M2-Deficient Mice Are Resistant to Acute Lung Injury Pubmed:35625931
Catalog No. Related products for research use of Bos taurus; Bovine (Cattle) Organism species Applications (RESEARCH USE ONLY!)
NPA780Bo01 Native Lactoferrin (LTF) Positive Control; Immunogen; SDS-PAGE; WB.
RPA780Bo01 Recombinant Lactoferrin (LTF) Positive Control; Immunogen; SDS-PAGE; WB.
APA780Bo02 Active Lactoferrin (LTF) Cell culture; Activity Assays.
APA780Bo01 Active Lactoferrin (LTF) Cell culture; Activity Assays.
PAA780Bo05 Polyclonal Antibody to Lactoferrin (LTF) WB
PAA780Bo01 Polyclonal Antibody to Lactoferrin (LTF) WB,ICC/IF
MAA780Bo21 Monoclonal Antibody to Lactoferrin (LTF) WB; IHC; ICC; IP.
SEA780Bo ELISA Kit for Lactoferrin (LTF) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA780Bo CLIA Kit for Lactoferrin (LTF) Chemiluminescent immunoassay for Antigen Detection.
LMA780Bo Multiplex Assay Kit for Lactoferrin (LTF) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA780Bo01 ELISA Kit DIY Materials for Lactoferrin (LTF) Main materials for "Do It (ELISA Kit) Yourself".