Active Pregnancy Associated Plasma Protein A (PAPPA)

PAPP-A; PAPA; ASBABP2; DIPLA1; IGFBP-4ase; PAPPA1; Pappalysin 1; Insulin-Like Growth Factor Binding Protein-4 Protease; Differentially Placenta 1 Expressed Protein

ACTIVITY TEST

Pregnancy-associated plasma protein A(PAPPA), also known as pappalysin-1, is a secreted protease whose main substrate is insulin-like growth factor binding proteins. PAPPA's proteolytic function is activated upon collagen binding. It is thought to be involved in local proliferative processes such as wound healing and bone remodeling. Low plasma level of this protein has been suggested as a biochemical marker for pregnancies with aneuploid fetuses (fetuses with an abnormal number of chromosomes). Besides, Plasminogen (Plg) has been identified as an interactor of PAPPA, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PAPPA and recombinant human Plg. Briefly, biotinylated PAPPA were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to Plg-coated microtiter wells and incubated for 1 h at 37℃. Wells were washed 3 times with PBST and incubated for 0.5 h at 37℃ with SA labelled HRP. After incubation with SA labelled HRP, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of PAPPA and Plg was shown in Figure 1, and the ED50 for this effect is 0.04936 ug/ml.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
American Journal of Physiology-Endocrinology and Metabolism Hyperandrogenism and insulin resistance induce gravid uterine defects in association with mitochondrial dysfunction and aberrant ROS production Pubmed: 30860876
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA800Hu03 Recombinant Pregnancy Associated Plasma Protein A (PAPPA) Positive Control; Immunogen; SDS-PAGE; WB.
RPA800Hu01 Recombinant Pregnancy Associated Plasma Protein A (PAPPA) Positive Control; Immunogen; SDS-PAGE; WB.
RPA800Hu05 Recombinant Pregnancy Associated Plasma Protein A (PAPPA) Positive Control; Immunogen; SDS-PAGE; WB.
APA800Hu01 Active Pregnancy Associated Plasma Protein A (PAPPA) Cell culture; Activity Assays.
APA800Hu02 Active Pregnancy Associated Plasma Protein A (PAPPA) Cell culture; Activity Assays.
APA800Hu03 Active Pregnancy Associated Plasma Protein A (PAPPA) Cell culture; Activity Assays.
RPA800Hu04 Recombinant Pregnancy Associated Plasma Protein A (PAPPA) Positive Control; Immunogen; SDS-PAGE; WB.
APA800Hu04 Active Pregnancy Associated Plasma Protein A (PAPPA) Cell culture; Activity Assays.
RPA800Hu02 Recombinant Pregnancy Associated Plasma Protein A (PAPPA) Positive Control; Immunogen; SDS-PAGE; WB.
PAA800Hu01 Polyclonal Antibody to Pregnancy Associated Plasma Protein A (PAPPA) WB; IHC; ICC; IP.
PAA800Hu02 Polyclonal Antibody to Pregnancy Associated Plasma Protein A (PAPPA) WB,IHC; FCM
PAA800Hu03 Polyclonal Antibody to Pregnancy Associated Plasma Protein A (PAPPA) WB; IHC; ICC; IP.
LAA800Hu71 Biotin-Linked Polyclonal Antibody to Pregnancy Associated Plasma Protein A (PAPPA) WB; IHC; ICC.
MAA800Hu21 Monoclonal Antibody to Pregnancy Associated Plasma Protein A (PAPPA) WB; IHC; ICC; IP.
MAA800Hu22 Monoclonal Antibody to Pregnancy Associated Plasma Protein A (PAPPA) WB; IHC; ICC; IP.
MAA800Hu24 Monoclonal Antibody to Pregnancy Associated Plasma Protein A (PAPPA) WB; IHC; ICC; IP.
SEA800Hu ELISA Kit for Pregnancy Associated Plasma Protein A (PAPPA) Enzyme-linked immunosorbent assay for Antigen Detection.
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