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Active Neuropeptide Y (NPY)

NP-Y; PYY4; CPON; Pro-neuropeptide Y; Neuropeptide tyrosine; C-flanking peptide of NPY

ACTIVITY TEST

Neuropeptide Y (NPY) belongs to the NPY family of biologically active peptides, which is implicated in the control of feeding and in secretion of gonadotrophin-release hormone. NPY was firstly isolated from brain extracts and found to be one of the most abundant neuropeptides within the brain. Significant release of NPY occurs especially upon stronger sympathetic activation, and recent data suggests that the importance of NPY seems enhanced in stress-related cardiovascular disorders. To test the effect of NPY on cell proliferation, MDA-MB-231 cells were seeded into triplicate wells of 96-well plates and allowed to attach, replaced with various concentrations of recombinant rat NPY. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 ℃. Cell viability was assessed by CCK-8 assay after incubation with recombinant rat NPY for 72h. The result was shown in Figure 1. It was obvious that NPY significantly decreased cell viability of MDA-MB-231 cells. The ED50 of recombinant rat NPY is 1.52 μg/ml.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Magazine Citations
Hemodialysis International Is there any interaction of resistin and adiponectin levels with protein‐energy wasting among patients with chronic kidney disease Onlinelibrary: hdi.12072
Journal of Pediatric Endocrinology and Metabolism Changes in growth pattern, leptin ghrelin and neuropeptide Y levels after adenotonsillectomy in prepubertal children Degruyter: Source
Ren Fail. The role of adipocytokines on depressive symptoms of patients with chronic kidney disease Pubmed: 23879728
Coronary Artery Disease The relationship of plasma neuropeptide Y levels with coronary collateral development. Pubmed: 24077326
Coronary Artery Disease The relationship of plasma neuropeptide Y levels with coronary collateral development Lww:Source
Toxicology Letters Possible role of serotonin and neuropeptide Y on the disruption of the reproductive axis activity by perfluorooctane sulfonate Pubmed:25623392
J Neurosurg Anesthesiol High Plasma Levels of Neuropeptide Y Correlate With Good Clinical Outcome But are not Correlated to Cerebral Blood Flow or Vasospasm After Subarachnoid Hemorrhage PubMed: 25955865
Journal of Diabetes Research High Intensity Aerobic Exercise Training Improves Deficits of Cardiovascular Autonomic Function in a Rat Model of Type 1 Diabetes Mellitus with Moderate … Pubmed:26885531
Archives of Gynecology and Obstetrics Binge eating and biochemical markers of appetite in new users of the contraceptive depot medroxyprogesterone acetate pubmed:27604241
basic & clinical pharmacology & toxicology Effects of Di-isononyl Phthalate on Neuropeptide Y Expression in Differentiating Human Neuronal Cells. pubmed:27625336
Acta Neurobiologiae Experimentalis Aberrant changes of somatostatin and neuropeptide Y in brain of a genetic rat model for epilepsy: tremor rat pubmed:27685769
Cardiovascular research Nur77 protects against adverse cardiac remodelling by limiting Neuropeptide Y signalling in the sympathoadrenal-cardiac axis Pubmed:29850786
Journal of Periodontology Salivary neuropeptides, stress, and periodontitis Pubmed:28914594
Neuropeptides Mechanisms of sustained long-term weight loss after RYGB: α-MSH is a key factor Pubmed:29685637
Pharmacology Melatonin Induces Osteoblastic Differentiation of Mesenchymal Stem Cells and Promotes Fracture Healing in a Rat Model of Femoral Fracture via Neuropeptide Y … Pubmed: 30227410
Marmara Medical Journal Investigation of the relationship between insulin resistance and neuropeptide Y levels in polycystic ovary syndrome
Marmara Medical Journal Investigation of the relationship between insulin resistance and neuropeptide Y levels in polycystic ovary syndrome.
neurochemical research Sevoflurane Induces Hippocampal Neuronal Apoptosis by Altering the Level of Neuropeptide Y in Neonatal Rats. Pubmed: 32378074
Hum Psychopharmacol Serum levels of neuropeptide Y in patients with chronic schizophrenia during treatment augmentation with sarcosine (results of the double©\blind randomized?¡­ 33245168
Arch Toxicol Serotonin involvement in okadaic acid-induced diarrhoea in vivo 34148100
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPA879Ra01 Recombinant Neuropeptide Y (NPY) Positive Control; Immunogen; SDS-PAGE; WB.
APA879Ra01 Active Neuropeptide Y (NPY) Cell culture; Activity Assays.
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PAA879Ra01 Polyclonal Antibody to Neuropeptide Y (NPY) WB; IHC; ICC; IP.
LAA879Ra71 Biotin-Linked Polyclonal Antibody to Neuropeptide Y (NPY) WB; IHC; ICC.
MAA879Ra21 Monoclonal Antibody to Neuropeptide Y (NPY) WB; IHC; ICC; IP.
CEA879Ra ELISA Kit for Neuropeptide Y (NPY) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA879Ra Multiplex Assay Kit for Neuropeptide Y (NPY) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA879Ra11 ELISA Kit DIY Materials for Neuropeptide Y (NPY) Main materials for "Do It (ELISA Kit) Yourself".