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Active Protein Tyrosine Phosphatase Receptor Type C (CD45)
PTPRC; PTPR-C; LCA; B220; GP180; LY5; T200; Leukocyte common antigen
- Product No.APB030Hu61
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 95%
- Isoelectric Point6.8
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 176
US$ 440
US$ 880
US$ 2640
US$ 6600
For more details, please contact local distributors!
ACTIVITY TEST
Protein Tyrosine Phosphatase Receptor Type C (CD45) is one of the most abundant leukocyte cell surface glycoproteins and is expressed exclusively upon cells of the hematopoietic system. CD45 functions positively to regulate lymphocyte activation by servesing to dephosphorylate and activate members of the Src-tyrosine kinase family. it has been reported that SEMA4D can associate with the phosphatase CD45 at the surface of T cells and that triggering of CD45 on T cells, using monoclonal antibodies (mAb), induces shedding of SEMA4D. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human CD45 and recombinant human SEMA4D. Briefly, biotin-linked CD45 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to SEMA4D-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µl stop solution to the wells and read at 450 nm immediately. The binding activity of CD45 and SEMA4D was shown in Figure 1, the EC50 for this effect is 6.47 ug/mL.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Magazine | Citations |
| Cell | Size-dependent segregation controls macrophage phagocytosis of antibody-opsonized targets Pubmed:29958103 |