≡

Active Caspase Activated DNase (CAD)

DFF-B; DFFB; CPAN; DFF40; DFF2; DNA Fragmentation Factor 40kDa Beta Polypeptide; Caspase-activated deoxyribonuclease; Caspase-activated nuclease

ACTIVITY TEST

Caspase Activated DNase (CAD), corresponding to product code RPB058Hu01, is a key nuclease in eukaryotic apoptosis. Normally, it exists in an inactive state in the cytoplasm, bound to its inhibitor. Upon apoptotic signal activation, effector caspases (e.g., Caspase-3) cleave the inhibitor, releasing active CAD. The activated enzyme translocates to the nucleus, where it cleaves chromosomal DNA into nucleosome-sized fragments, a hallmark of apoptotic cell death. Its strict regulation prevents unintended DNA damage, ensuring genomic stability in healthy cells. CAD interacts with DFFa (product code L426Hu) as its specific inhibitor; DFFa binds CAD to inhibit its nuclease activity in non-apoptotic cells.Thus a functional ELISA assay was conducted to detect the interaction of recombinant human CAD and recombinant human DFFa. Briefly, CAD was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to DFFa-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CAD pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human CAD and recombinant human DFFa was shown in Figure 1, the EC50 for this effect is 0.01067µg/mL.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Cell Discovery A novel role for synaptic acetylcholinesterase as an apoptotic deoxyribonuclease Articles: Celldisc20152
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB058Hu01 Recombinant Caspase Activated DNase (CAD) Positive Control; Immunogen; SDS-PAGE; WB.
APB058Hu01 Active Caspase Activated DNase (CAD) Cell culture; Activity Assays.
PAB058Hu01 Polyclonal Antibody to Caspase Activated DNase (CAD) WB; IHC; ICC; IP.
MAB058Hu22 Monoclonal Antibody to Caspase Activated DNase (CAD) WB; IHC; ICC; IP.
SEB058Hu ELISA Kit for Caspase Activated DNase (CAD) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB058Hu Multiplex Assay Kit for Caspase Activated DNase (CAD) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.