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Active Galanin (GAL)

GALN; GLNN; GMAP; Galanin Prepropeptide; Galanin-Message-Associated Peptide

ACTIVITY TEST

Galanin (GAL) is a neuropeptide widely distributed in the central and peripheral nervous systems. It consists of 29 or 30 amino acids and is involved in various physiological processes like regulating appetite, pain sensation, memory, and endocrine function.Besides,Galanin Like Peptide (GALP) has been identified as an interactor of GAL, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human GAL and recombinant rat GALP. Briefly, biotin-linked GAL were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to GALP-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of GAL and GALP was shown in Figure 1, the EC50 for this effect is 0.282 ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Progress in Neuro-Psychopharmacology and Biological Psychiatry Decreased galanin serum levels are associated with alcohol-craving during withdrawal ScienceDirect: S0278584610005191
Clinical Biochemistry Circulating galanin levels are increased in patients with gestational diabetes mellitus PubMed: 23266294
Peptides Endogenous galanin as a novel biomarker to predict gestational diabetes mellitus. Pubmed: 24503374
Gynecological Endocrinology Circulating galanin and IL-6 concentrations in gestational diabetes mellitus Pubmed:24397394
Eat Behav Association between circulating levels of galanin and pre-pregnancy body mass index in patients with gestational diabetes mellitus PubMed: 26172564
Cell Biochemistry and Biophysics The Effects of Levetiracetam on Cerebrospinal Fluid and Plasma NPY and GAL, and on the Components of Stress Response System, hs-CRP, and S100B Protein in Serum of Patients with Refractory Epilepsy Article: 10.1007
Journal of Addiction & Prevention Low Galanin Serum Levels are Associated with Anxiety in Opioid-Maintained Patients 688687ca8d5a1d03ed060f761328001ca673.pdf
Oncology Letter Colorectal cancer patients exhibit increased levels of galanin in serum and colon tissues pubmed:27899999
Scientific Reports Serum Galanin Levels in Young Healthy Lean and Obese Non-Diabetic Men during an OralGlucose Tolerance Test. pubmed:27550417
Journal of Clinical Investigation  Adrenomedullin and Galanin Responses to Orthostasis in Older Persons pubmed:28796366
Child Psychiatry Hum Dev Serum Galanin in Children with Autism Spectrum Disorder 33534034
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
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