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Active Perforin 1 (PRF1)

FLH2; HPLH2; P1; PFP; Pore Forming Protein; Cytolysin; Lymphocyte pore-forming protein

ACTIVITY TEST

Perforin 1 (PRF1) is a cytolytic protein found in the granules of Cytotoxic T lymphocytes (CTLs) and NK cells. Upon degranulation, perforin inserts itself into the target cell's plasma membrane, forming a pore. The lytic membrane-inserting part of perforin is the MACPF domain. This region shares homology with cholesterol-dependent cytolysins from Gram-positive bacteria. It has been demonstrated that PRF1 causes the lysis of abnormal body cells and the elimination of virus-infected cells and tumors.To test the effect of PRF1 on cell apoptosis，Hela cells were seeded into triplicate wells of 96-well plates and allowed to attach, replaced with various concentrations of recombinant mouse PRF1. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 ℃. Cell viability was assessed by CCK-8 assay after incubation with recombinant mouse PRF1 for 72h. The result was shown in Figure 1. It was obvious that PRF1 significantly decreased cell viability of Hela cells. The ED50 of recombinant mouse PRF1 is 3.205 μg/ml.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Diagnostic Microbiology and Infectious Disease Granzyme B as a diagnostic marker of tuberculosis in patients with and without HIV coinfection Pubmed:26915636
FASEB JOURNAL The immunoregulatory effects of CD8 T-cell–derived perforin on diet-induced nonalcoholic steatohepatitis Pubmed: 30951375
Theranostics A novel Granzyme B nanoparticle delivery system simulates immune cell functions for suppression of solid tumors Pubmed: 31695790
Mol Immunol CD317 mediates immunocytolysis resistance by RICH2/cytoskeleton-dependent membrane protection Pubmed: 33223223
INVESTIGATIONAL NEW DRUGS Dual-function chimeric antigen receptor T cells targeting c-Met and PD-1 exhibit potent anti-tumor efficacy in solid tumors Pubmed: 32772342
Am J Transl Res Combined treatment with epigenetic agents enhances anti-tumor activity of T cells by upregulating the ACRBP expression in hepatocellular carcinoma 34377237
Nat Biomed Eng Cancer-cell stiffening via cholesterol depletion enhances adoptive T-cell immunotherapy 34873307
J Exp Med Single-cell immune profiling reveals functional diversity of T cells in tuberculous pleural effusion Pubmed:35061012
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPB317Mu01 Recombinant Perforin 1 (PRF1) Positive Control; Immunogen; SDS-PAGE; WB.
RPB317Mu02 Recombinant Perforin 1 (PRF1) Positive Control; Immunogen; SDS-PAGE; WB.
APB317Mu01 Active Perforin 1 (PRF1) Cell culture; Activity Assays.
APB317Mu03 Active Perforin 1 (PRF1) Cell culture; Activity Assays.
APB317Mu02 Active Perforin 1 (PRF1) Cell culture; Activity Assays.
RPB317Mu03 Recombinant Perforin 1 (PRF1) Positive Control; Immunogen; SDS-PAGE; WB.
PAB317Mu01 Polyclonal Antibody to Perforin 1 (PRF1) WB
PAB317Mu02 Polyclonal Antibody to Perforin 1 (PRF1) WB; IHC; ICC; IP.
LAB317Mu81 FITC-Linked Polyclonal Antibody to Perforin 1 (PRF1) WB; IHC; ICC; IF.
LAB317Mu71 Biotin-Linked Polyclonal Antibody to Perforin 1 (PRF1) WB; IHC; ICC.
WEB317Mu Wide-range ELISA Kit for Perforin 1 (PRF1) Enzyme-linked immunosorbent assay for Antigen Detection.
SEB317Mu ELISA Kit for Perforin 1 (PRF1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB317Mu Multiplex Assay Kit for Perforin 1 (PRF1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.