Active B-Lymphocyte Activation Antigen B7-1 (LAB7-1)
CD80; CD28LG; CD28LG1; BB1; B7-1 Antigen; T-lymphocyte activation antigen CD80; CTLA-4 counter-receptor B7.1
- Product No.APB386Hu61
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 95%
- Isoelectric Point5.4
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
For more details, please contact local distributors! 460 US$ 460
For more details, please contact local distributors! US$ 920
For more details, please contact local distributors! US$ 2760
For more details, please contact local distributors! US$ 6900
For more details, please contact local distributors!
B-Lymphocyte Activation Antigen B7-1 (CD80) is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. CD80 can combine with CD86, thus, a binding ELISA assay was conducted to detect the association of recombinant human CD80 with recombinant human CD86. Briefly, biotin-linked recombinant human CD80 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CD86-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µl stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human CD80 and recombinant human CD86 was shown in Figure 1, the EC50 for this effect is 0.412 ug/mL.
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- BCA Protein Quantification Kit
- Molecular Mass Marker for Protein
- Monoclonal Antibody Customized Service
- Polyclonal Antibody Customized Service
- Protein Activity Test Experiment Service
- Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
- Lentivirus Packaging Experiment Service
- Adenovirus Packaging Experiment Service
- Real Time PCR Experimental Service
- Spike RBD Protein (S-RBD)
- Protein G
- Protein A
|BioMed Research International||Predictability of Urinary CD80 in the Relapse of Primary Nephrotic Syndrome pubmed:28951877|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|EPB386Hu61||Eukaryotic B-Lymphocyte Activation Antigen B7-1 (LAB7-1)||Positive Control; Immunogen; SDS-PAGE; WB.|
|APB386Hu61||Active B-Lymphocyte Activation Antigen B7-1 (LAB7-1)||Cell culture; Activity Assays.|
|PAB386Hu01||Polyclonal Antibody to B-Lymphocyte Activation Antigen B7-1 (LAB7-1)||WB; IHC; ICC; IP.|
|SEB386Hu||ELISA Kit for B-Lymphocyte Activation Antigen B7-1 (LAB7-1)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|LMB386Hu||Magnetic Luminex Assay Kit for B-Lymphocyte Activation Antigen B7-1 (LAB7-1) ,etc.||Magnetic Luminex Assay for Antigen Detection.|