≡

Active Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF)

HB-EGF; DTR; DTS; DTSF; HEGFL; Diphtheria Toxin Receptor; Proheparin-binding EGF-like growth factor; Diphtheria toxin receptor

ACTIVITY TEST

Heparin-binding Epidermal Growth Factor-like Growth Factor (HBEGF) is a member of the epidermal growth factor (EGF) family. As a ligand for the EGF receptor (EGFR), HBEGF can bind to both 170 kDa EGFR and 180 kDa ErbB4, and through heterodimerization, ErbB2. HBEGF protein involves in several processes, including epidermal growth factor receptor signaling pathway，phosphatidylinositol 3-kinase/protein kinase B signal transduction and wound healing. To test the effect of HBEGF on cell proliferation, BALB/3T3 cells were seeded into triplicate wells of 96-well plates and allowed to attach, replaced with various concentrations of recombinant human HBEGF. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 ℃. Cell viability was assessed by CCK-8 assay after incubation with recombinant human HBEGF for 72h. The result was shown in Figure 1. It was obvious that HBEGF significantly increased cell viability of BALB/3T3 cells. The ED50 of recombinant human HBEGF is 1.033μg/ml.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Neuropathology Astrocyte ERK phosphorylation precedes K+-induced swelling but follows hypotonicity-induced swelling. PubMed: 21118399
Toxicological Sciences Cypermethrin Induces Astrocyte Apoptosis by the Disruption of the Autocrine/Paracrine Mode of Epidermal Growth Factor Receptor Signaling Pubmed: source
PAIN RESEARCH 軽度炎症性膀胱痛マウスモデルの構築とNGFの関与 Jstage:Source
European Journal of Pain A novel method for assessing bladder-related pain reveals the involvement of nerve growth factor in painassociated with cyclophosphamide-induced chronic cystitis in mice. Pubmed:25820250
Kinki daigaku 2 型糖尿病モデルラットにおける糖尿病性腎症の進行とHB-EGF の関連性 17844
laboratory investigation Heparin-binding epidermal growth factor contributes to COPD disease severity by modulating airway fibrosis and pulmonary epithelial–mesenchymal transition Pubmed:29581578
International Journal of Biological Sciences CD9 regulates keratinocyte migration by negatively modulating the sheddase activity of ADAM17
Redox Biology Tumoral NOX4 recruits M2 tumor-associated macrophages via ROS/PI3K signaling-dependent various cytokine production to promote NSCLC growth Pubmed: 30769285
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Polarization of ADAM17‐driven EGFR signalling in electric field‐guided collective migration of epidermal sheets Pubmed: 33164313
象牙芽細胞の枯渇は象牙芽細胞分化と象牙質形成を誘導する
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APB479Hu01 Active Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) Cell culture; Activity Assays.
APB479Hu61 Active Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) Cell culture; Activity Assays.
EPB479Hu62 Eukaryotic Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) Positive Control; Immunogen; SDS-PAGE; WB.
APB479Hu62 Active Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) Cell culture; Activity Assays.
EPB479Hu61 Eukaryotic Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) Positive Control; Immunogen; SDS-PAGE; WB.
RPB479Hu01 Recombinant Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) Positive Control; Immunogen; SDS-PAGE; WB.
PAB479Hu01 Polyclonal Antibody to Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) WB; IHC
LAB479Hu71 Biotin-Linked Polyclonal Antibody to Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) WB; IHC; ICC.
MAB479Hu22 Monoclonal Antibody to Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) WB
MAB479Hu25 Monoclonal Antibody to Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) ICC/IF
MAB479Hu21 Monoclonal Antibody to Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) IHC
MAB479Hu23 Monoclonal Antibody to Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) IHC
MAB479Hu24 Monoclonal Antibody to Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) IHC
SEB479Hu ELISA Kit for Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB479Hu CLIA Kit for Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) Chemiluminescent immunoassay for Antigen Detection.
LMB479Hu Multiplex Assay Kit for Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.