≡

Active Trefoil Factor 3 (TFF3)

HITF; ITF3; TFI; HP1.B; Trefoil Factor 3, Intestinal

ACTIVITY TEST

Trefoil Factor 3 (TFF3) is a small secretory protein primarily produced by gastrointestinal goblet cells, belonging to the trefoil factor family (TFF) characterized by a conserved trefoil domain. It plays crucial roles in mucosal protection, epithelial repair, and inflammation regulation by resisting protease degradation and promoting cell migration. TFF3 is highly expressed in the intestines and colon, where it stabilizes the mucus layer and facilitates wound healing. Additionally, TFF3 directly binds to FCGBP to form a heteromeric complex that enhances mucosal stability and repair.To detect the activity of recombinant TFF3, a functional ELISA assay was performed to evaluate the interaction between recombinant rat TFF3 and recombinant human FCGBP.Briefly, biotin-linked TFF3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100µl were then transferred to FCGBP-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of TFF3 and FCGBP was shown in Figure 1, the EC50 for this effect is 0.21µg/mL..

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
BMC Gastroenterology Serum trefoil factor 3 is a promising non-invasive biomarker for gastric cancer screening: A monocentric cohort study in China Biomedcentral: Source
Technology in Cancer Research & Treatment Serum Trefoil Factor 3 as a Protein Biomarker for the Diagnosis of Colorectal Cancer. pubmed:27760866
PLoS One Obstructive sleep apnea and rhonchopathy are associated with downregulation of trefoil factor family peptide 3 (TFF3)-Implications of changes in oral mucus composition. pubmed:29028798
Microbial Pathogenesis Protective effects of sinomenine against LPS-induced inflammation in piglets. pubmed:28757275
Scientific Reports Nucleotide-mediated SPDEF modulates TFF3-mediated wound healing and intestinal barrier function during the weaning process Pubmed:29555969
Scientific Reports Kidney-based in vivo model for drug-induced nephrotoxicity testing Pubmed: 32796873
International Journal of Molecular Sciences Human Synovia Contains Trefoil Factor Family (TFF) Peptides 1–3 Although Synovial Membrane Only Produces TFF3: Implications in Osteoarthritis and Rheumatoid …
Int. J. Mol. Sci. Untersuchungen der Trefoil factor family Peptide 1-3 in humaner Synovialfl¨¹ssigkeit und Synovialmembran und deren Bedeutung bei Arthrose und?¡­
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
APB656Ra61 Active Trefoil Factor 3 (TFF3) Cell culture; Activity Assays.
EPB656Ra61 Eukaryotic Trefoil Factor 3 (TFF3) Positive Control; Immunogen; SDS-PAGE; WB.
RPB656Ra01 Recombinant Trefoil Factor 3 (TFF3) Positive Control; Immunogen; SDS-PAGE; WB.
CPB656Ra21 OVA Conjugated Trefoil Factor 3 (TFF3) Immunogen; SDS-PAGE; WB.
PAB656Ra08 Polyclonal Antibody to Trefoil Factor 3 (TFF3) Testing in progress.
PAB656Ra01 Polyclonal Antibody to Trefoil Factor 3 (TFF3) WB; IHC; ICC/IF
PAB656Ra02 Polyclonal Antibody to Trefoil Factor 3 (TFF3) WB; IHC; ICC; IP.
LAB656Ra71 Biotin-Linked Polyclonal Antibody to Trefoil Factor 3 (TFF3) WB; IHC; ICC.
MAB656Ra21 Monoclonal Antibody to Trefoil Factor 3 (TFF3) WB; IHC; ICC; IP.
SEB656Ra ELISA Kit for Trefoil Factor 3 (TFF3) Enzyme-linked immunosorbent assay for Antigen Detection.
HEB656Ra High Sensitive ELISA Kit for Trefoil Factor 3 (TFF3) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB656Ra Multiplex Assay Kit for Trefoil Factor 3 (TFF3) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB656Ra01 ELISA Kit DIY Materials for Trefoil Factor 3 (TFF3) Main materials for "Do It (ELISA Kit) Yourself".